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 >  Protein>CD7 >CD7-H82E4

Biotinylated Human CD7 Protein, His,Avitag™, premium grade

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利用生物素-链霉亲和素系统的高特异性和高灵敏度的结合特性,可将生物素化蛋白与链霉亲和素相关产品联合应用于各类实验,与非生物素体系相比,检测速度更快、结合能力更强、非特异背景更低。

分子别名(Synonym)

CD7,GP40,TP41,LEU-9,Tp40

表达区间及表达系统(Source)

Biotinylated Human CD7 Protein, His,Avitag, premium grade (CD7-H82E4) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Pro 180 (Accession # P09564-1 ).

Predicted N-terminus: Ala 26

It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.

Request for sequence

蛋白结构(Molecular Characterization)

CD7 Structure

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 20.0 kDa. The protein migrates as 28-38 kDa under reducing (R) condition, and 26-36 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 0.01 EU per μg by the LAL method.

宿主蛋白残留(Host Cell Protein)

<0.5 ng/µg of protein tested by ELISA.

宿主核酸残留(Host Cell DNA)

<0.02 ng/μg of protein tested by qPCR.

无菌(Sterility)

The sterility testing was performed by membrane filtration method.

支原体(Mycoplasma)

Negative.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 24 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

CD7 SDS-PAGE

Biotinylated Human CD7 Protein, His,Avitag, premium grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS

CD7 SEC-MALS

The purity of Biotinylated Human CD7 Protein, His,Avitag, premium grade (Cat. No. CD7-H82E4) is more than 90% and the molecular weight of this protein is around 52-62 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

CD7 ELISA

Immobilized Anti-CD7 antibody, Mouse IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human CD7 Protein, His,Avitag, premium grade (Cat. No. CD7-H82E4) with a linear range of 0.5-8 ng/mL (QC tested).

Protocol

CD7 ELISA

Immobilized Biotinylated Human CD7 Protein, His,Avitag, premium grade (Cat. No. CD7-H82E4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-CD7 antibody, Mouse IgG1 with a linear range of 0.1-2 ng/mL (Routinely tested).

Protocol

 

背景(Background)

T-cell antigen CD7 (CD7) is also known as GP40, LEU-9, TP41 and Tp40. CD7 is a protein that in humans is encoded by the CD7 gene, this gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. CD7 has been shown to interact with PIK3R1. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development.

 

前沿进展

 
 
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CD7靶点信息
英文全称:T-cell antigen CD7
中文全称:T细胞抗原CD7
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:23详情
最高研发阶段:临床二期
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