登录 | 注册    关注公众号  
微信公众号
搜索
 >  Protein>B7-H3 (4Ig) >B7B-HF2E7

FITC-Labeled Human B7-H3 (4Ig) / B7-H3b Protein, His Tag (MALS verified)

分子别名(Synonym)

4Ig-B7-H3,B7-H3,CD276,PSEC0249,UNQ309,PRO352,B7 homolog 3

表达区间及表达系统(Source)

FITC-Labeled Human B7-H3 (4Ig), His Tag (B7B-HF2E7) is expressed from human 293 cells (HEK293). It contains AA Gly 27 - Thr 461 (Accession # Q5ZPR3-1 ). It is the FITC labeled form of Human B7-H3 (4Ig), His Tag (Cat. No.B7B-H52E7).

Predicted N-terminus: Gly 27

Request for sequence

蛋白结构(Molecular Characterization)

B7-H3 (4Ig) Structure

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 48.5 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

偶联(Conjugate)

FITC

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 535 nm

标记(Labeling)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.

蛋白标记度(Protein Ratio)

The FITC to protein molar ratio is 1-2.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

B7-H3 (4Ig) SDS-PAGE

FITC-Labeled Human B7-H3 (4Ig), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

B7-H3 (4Ig) SEC-MALS

The purity of FITC-Labeled Human B7-H3 (4Ig), His Tag (Cat. No. B7B-HF2E7) is more than 90% and the molecular weight of this protein is around 55-75 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

B7-H3 (4Ig) ELISA

Immobilized Monoclonal Anti-Human B7-H3 / B7-H3 (4Ig) Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind FITC-Labeled Human B7-H3 (4Ig), His Tag (Cat. No. B7B-HF2E7) with a linear range of 0.039-0.156 μg/mL (QC tested).

Protocol

 

活性(Bioactivity)-FACS

B7-H3 (4Ig) FACS

2e5 of anti-B7-H3 CAR-293 cells were stained with 100 μL of 3 μg/mL of FITC-Labeled Human B7-H3 (4Ig) , His Tag (Cat. No. B7B-HF2E7) and negative control protein respectively, FITC signals was used to evaluate the binding activity (QC tested).

Protocol

 

背景(Background)

Human B7 homolog 3 (B7-H3) is a member of the B7 family of immune proteins that provide signals for the regulation of immune responses. Other family members include B7-1, B7-2, B7-H1/PD-L1, B7-H2, and PD-L2. B7 family proteins are type I transmembrane immunoglobulin (Ig) superfamily members that contain extracellular Ig V‑like and Ig C‑like domains with a short cytoplasmic tail. Termed 4IgB7-H3 or B7-H3b, this molecule has two additional Ig-like domains (one V‑type and one C‑type) and shows a ubiquituous expression pattern.

 

前沿进展

 
 
货号/价格
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
B7-H3 (4Ig)靶点信息
英文全称:CD276 antigen
中文全称:CD276抗原
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:35详情
最高研发阶段:申请上市
查看更多信息
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定