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 >  Protein>IL-22 >IL2-H8247

Biotinylated Human IL-22 Protein, His,Avitag™

分子别名(Synonym)

IL-22,IL-TIF,ZCYTO18

表达区间及表达系统(Source)

Biotinylated Human IL-22, His,Avitag (IL2-H8247) is expressed from human 293 cells (HEK293). It contains AA Ala 34 - Ile 179 (Accession # Q9GZX6-1).

Predicted N-terminus: His

Request for sequence

蛋白结构(Molecular Characterization)

IL-22 Structure

This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™)

The protein has a calculated MW of 20.3 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

IL-22 SDS-PAGE

Biotinylated Human IL-22, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

IL-22 ELISA

Immobilized Biotinylated Human IL-22, His,Avitag (Cat. No. IL2-H8247) at 1 μg/mL (100 μL/well)on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate. can bind Human IL-22 Antibody with a linear range of 0.1-4 ng/mL (QC tested).

Protocol

IL-22 ELISA

Immobilized Human IL-22 Antibody at 1 μg/mL (100 μL/well) can bind Biotinylated Human IL-22, His,Avitag (Cat. No. IL2-H8247) with a linear range of 1-31 ng/mL (Routinely tested).

Protocol

IL-22 ELISA

Immobilized Biotinylated Human IL-22, His,Avitag (Cat. No. IL2-H8247) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human IL-22 R alpha 1, Fc Tag (Cat. No. IL1-H5258) with a linear range of 0.6-78 ng/mL (Routinely tested).

Protocol

IL-22 ELISA

Immobilized Human IL-22BP Protein, Fc Tag (Cat. No. ILP-H5254) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-22, His,Avitag (Cat. No. IL2-H8247) with a linear range of 0.03-1 ng/mL (Routinely tested).

Protocol

 

背景(Background)

Interleukin-22 (IL22) is also known as cytokine Zcyto18, IL-10-related T-cell-derived-inducible factor (IL-TIF), which is belongs to the IL-10 family or IL-10 superfamily (including IL-19, IL-20, IL-24, and IL-26), a class of potent mediators of cellular inflammatory responses. IL-22 is produced by activated DC and T cells and initiates innate immune responses against bacterial pathogens especially in epithelial cells such as respiratory and gut epithelial cells. IL-22 biological activity is initiated by binding to a cell-surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein IL-22BP. IL-22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10.

 

前沿进展

 
 
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IL-22靶点信息
英文全称:Interleukin-22
中文全称:白细胞介素-22
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:2详情
最高研发阶段:临床二期
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