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 >  Protein>ENPP3 >EN3-H52H4

Human ENPP3 / CD203c Protein, His Tag (active enzyme)

分子别名(Synonym)

ENPP3,CD203c,NPP3,E-NPP3,PD-Ibeta,NPPase,PDNP3

表达区间及表达系统(Source)

Human ENPP3, His Tag (EN3-H52H4) is expressed from human 293 cells (HEK293). It contains AA Leu 48 - Ile 875 (Accession # O14638-1).

Predicted N-terminus: His

Request for sequence

蛋白结构(Molecular Characterization)

Online(Leu 48 - Ile 875) O14638-1

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 96.9 kDa. The protein migrates as 115-120 kDa and 121-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human ENPP3, His Tag (Cat. No. EN3-H52H4) SDS-PAGE gel

Human ENPP3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)

Measured by its ability to hydrolyze the Pyrophosphate (PPi) from the substrate adenosine-5’-triphosphate (ATP). The specific activity is > 150 pmol/min/μg (QC tested).

Protocol

 

背景(Background)

The human NPP family contains seven members which can be classified into two groups according to their substrate preferences. The first class comprises the nucleotide-degrading proteins NPP1, 3 and 4. NPP3 (CD203c, ENPP3) is expressed in multiple organs, including on epithelial and mucosal surfaces, and notably on basophils and mast cells. Activation of basophils by antigen-bound IgE leads to release of inflammatory mediators and rapid upregulation of NPP3 to the cell surface. This protein is in fact a common marker for diagnosing allergen sensitivity with patient basophils by flow cytometry. Basophils and mast cells mediate the response to certain pathogens, as well as acute and chronic allergic reactions. Following activation, these cells release ATP, which further stimulates them in an autocrine manner. NPP3 upregulation serves to degrade ATP and suppress chronic allergic inflammation (but not the acute response).

 

 

前沿进展

 
 
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ENPP3靶点信息
英文全称:Ectonucleotide pyrophosphatase/phosphodiesterase family member 3
中文全称:核苷酸内焦磷酸酶/磷酸二酯酶家族成员-3
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:3详情
最高研发阶段:临床二期
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