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 >  Protein>Nectin-4 >NE4-HF2H3

FITC-Labeled Human Nectin-4 Protein, His Tag

分子别名(Synonym)

NECTIN4,LNIR, PRR4, PVRL4

表达区间及表达系统(Source)

FITC-Labeled Human Nectin-4, His Tag (NE4-HF2H3) is expressed from human 293 cells (HEK293). It contains AA Gly 32 - Ser 349 (Accession # Q96NY8-1). It is the FITC labeled form of Human Nectin-4, His Tag (NE4-H52H3).

Predicted N-terminus: Gly 32

Request for sequence

蛋白结构(Molecular Characterization)

Online(Gly 32 - Ser 349) Q96NY8-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 36.0 kDa. The protein migrates as 38-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Conjugate

FITC

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 535 nm

标记(Labeling)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.

蛋白标记度(FITC:Protein Ratio)

The FITC to protein molar ratio is 1-3.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

FITC-Labeled Human Nectin-4, His Tag (Cat. No. NE4-HF2H3) SDS-PAGE gel

FITC-Labeled Human Nectin-4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Anti-Nectin-4 Antibody, Mouse IgG1 at 2 μg/mL (100 μL/well) can bind FITC-Labeled Human Nectin-4, His Tag (Cat. No. NE4-HF2H3) with a linear range of 10-78 ng/mL (QC tested).

Protocol

活性(Bioactivity)-FACS

Biotinylated Human FACS

2e5 of anti-Nectin-4 CAR-293 cells were stained with 100 μL of 10 μg/mL of FITC-Labeled Human Nectin-4, His Tag (Cat. No. NE4-HF2H3) and negative control protein respectively, FITC signal was used to evaluate the binding activity (QC tested).

Protocol

 

背景(Background)

Nectin-4 (gene name PVRL4, poliovirus receptor-like 4) is a 66 kDa type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. Nectins are cell adhesion molecules that play a key role in various biological processes such as polarity, proliferation, differentiation and migration, for epithelial, endothelial, immune and neuronal cells, during development and adult life. Nectin-4 is a tumor-associated antigen in 50%, 49% and 86%o of breast, ovarian and lung carcinomas, respectively, mostly on tumors of bad prognosis. Its expression is not detected in the corresponding normal tissues.

 

 

前沿进展

 
 
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Nectin-4靶点信息
英文全称:Nectin-4
中文全称:细胞黏附分子nectin-4
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:8详情
最高研发阶段:批准上市
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