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 >  Protein>ASGR1 >AS1-H5253

Human ASGR1 / ASGPR1 Protein, Fc Tag (MALS verified)

分子别名(Synonym)

ASGP-R 1, ASGPR 1, C-type lectin domain family 4 member H1, Hepatic lectin H1, HL-1

表达区间及表达系统(Source)

Human ASGR1, Fc Tag (AS1-H5253) is expressed from human 293 cells (HEK293). It contains AA Gln 62 - Leu 291 (Accession # P07306-1 ).

Predicted N-terminus: Pro

Request for sequence

蛋白结构(Molecular Characterization)

ASGR1 Structure

This protein carries a human IgG1 Fc tag at the N-terminus.

The protein has a calculated MW of 52.8 kDa. The protein migrates as 65-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

ASGR1 SDS-PAGE

Human ASGR1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

ASGR1 SEC-MALS

The purity of Human ASGR1, Fc Tag (Cat. No. AS1-H5253) is more than 85% and the molecular weight of this protein is around 105-125 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-BLI

ASGR1 BLI

Loaded Human ASGR1, Fc Tag (Cat. No. AS1-H5253) on Protein A Biosensor, can bind SARS-CoV-2 S1 protein NTD, His Tag (Cat. No. S1D-C52H6) with an affinity constant of 401 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

ASGR1 BLI

Loaded Human ASGR1, Fc Tag (Cat. No. AS1-H5253) on Protein A Biosensor, can bind SARS-CoV-2 S1 protein with an affinity constant of 4.09 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

ASGR1 BLI

Loaded Human ASGR1, Fc Tag (Cat. No. AS1-H5253) on Protein A Biosensor, can bind SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) with an affinity constant of 686 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

ASGPR, a transmembrane C-type lectin, recognizes a wide variety of ligands that contain either terminal galactose (Gal) or N-acetylgalactosamine (GalNAc) residues and has been identified as a highly selective receptor on hepatocytes. The ASGR is composed of both a major (ASGR1) and minor subunit (ASGR2). ASGR1 has been shown to be efficiently targeted to the plasma membrane and to undergo constitutive endocytosis and recycling and found mainly expressed in the human liver.

 

前沿进展

 
 
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ASGR1靶点信息
英文全称:Asialoglycoprotein receptor 1
中文全称:去唾液酸糖蛋白受体-1
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:0详情
最高研发阶段:临床前
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