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Human IL-2 R beta&IL-2 R alpha&IL-2 R gamma Protein, Fc Tag&Fc Tag (MALS verified)

分子别名(Synonym)

IL-2 R beta & IL-2 R alpha & IL-2 R gamma

表达区间及表达系统(Source)

Human IL-2RB&IL-2RA&IL-2RG, Fc Tag&Fc Tag (ILG-H5257) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Asp 239 & Glu 22 - Ser 212 & Leu 23 - Asn 254 (Accession # P14784-1(IL-2RB) & P01589-1(IL-2RA) & P31785-1(IL-2RG)).

Predicted N-terminus: Ala 27 & Glu 22

蛋白结构(Molecular Characterization)

Human IL-2RB&IL-2RA&IL-2RG, Fc Tag&Fc Tag is produced by co-expression of IL-2RB and IL-2RA and IL-2RG, has a calculated MW of 51.1 kDa (IL-2RB) and 75.4 kDa (IL-2RA&IL-2RG). Subunit IL-2RB is fused with a human IgG1 Fc tag at the C-terminus and subunit IL-2RA&IL-2RG is fused with a human IgG1 Fc tag at the C-terminus. The reducing (R) protein migrates as 60-66 kDa and 100-116 kDa respectively due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human IL-2RB&IL-2RA&IL-2RG, Fc Tag&Fc Tag (Cat. No. ILG-H5257) SDS-PAGE gel

Human IL-2RB&IL-2RA&IL-2RG, Fc Tag&Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Human IL-2RB&IL-2RA&IL-2RG, Fc Tag&Fc Tag (Cat. No. ILG-H5257) MALS images

The purity of Human IL-2RB&IL-2RA&IL-2RG, Fc Tag&Fc Tag(Cat. No. ILG-H5257) was more than 90% and the molecular weight of this protein is around 175-190 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Biotinylated Human IL-2, His,Avitag (Cat. No. IL2-H82E4) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human IL-2RB&IL-2RA&IL-2RG, Fc Tag&Fc Tag (Cat. No. ILG-H5257) with a linear range of 0.4-6 ng/mL (QC tested).

Protocol

活性(Bioactivity)-SPR

Biotinylated Human SPR

Human IL-2RB&IL-2RA&IL-2RG, Fc Tag&Fc Tag (Cat. No. ILG-H5257) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-2, Tag Free (Cat. No. IL2-H4113) with an affinity constant of 40.6 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Protocol

 

背景(Background)

Both Interleukin-2 receptor subunit beta and Interleukin-2 receptor subunit gamma are receptor for interleukin-2. Common subunit for the receptors for a variety of interleukins. Interacts with SHB upon interleukin stimulation. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.

 

前沿进展

 
 
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IL-2 R beta & IL-2 R alpha & IL-2 R gamma靶点信息
英文全称:Interleukin-2
中文全称:白细胞介素-2
种类:Homo sapiens
上市药物数量:2详情
临床药物数量:10详情
最高研发阶段:批准上市
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