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SARS-CoV-2 S protein, His Tag, Super stable trimer (MALS verified)

分子别名(Synonym)

Spike,S protein,Spike glycoprotein,S glycoprotein

表达区间及表达系统(Source)

SARS-CoV-2 S protein, His Tag, Super stable trimer (SPN-C52H7) is expressed from human 293 cells (HEK293).

Predicted N-terminus: Val 16

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 138.0 kDa. The protein migrates as 170-250 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Supplied as 0.2 μm filtered solution in PBS.

Contact us for customized product form or formulation.

运输(Shipping)

This product is supplied and shipped as sterile liquid solution with dry ice, please inquire the shipping cost.

存储(Storage)

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. The product MUST be stored at -70°C or lower upon receipt;
  2. -70°C for 3 months under sterile conditions.
 

电泳(SDS-PAGE)

SARS-CoV-2 S protein, His Tag, Super stable trimer (Cat. No. SPN-C52H7) SDS-PAGE gel

SARS-CoV-2 S protein, His Tag, Super stable trimer on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

SARS-CoV-2 S protein, His Tag, Super stable trimer (Cat. No. SPN-C52H7) MALS images

The purity of SARS-CoV-2 S protein, His Tag, Super stable trimer (Cat. No. SPN-C52H7) is more than 90% and the molecular weight of this protein is around 480-550 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein, His Tag, Super stable trimer (Cat. No. SPN-C52H7) with a linear range of 0.2-6 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Anti-SARS-CoV-2 RBD Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein, His Tag, Super stable trimer (Cat. No. SPN-C52H7) with a linear range of 0.2-3 ng/mL (Routinely tested).

Protocol

背景(Background)

It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

 

前沿进展

 
 

Purchaser Notification

Important Licensing Information: This product may be covered by Limited Use Label License(s). By use of this product, you accept the terms and conditions of all applicable Limited Use Label License(s).

Limited Use Label License No. 18: Engineered Coronavirus Spike Proteins

This product and its use is the subject of U.S. Provisional Patent Application No. 63/032,502 and sold under a license from The University of Texas at Austin. Rights to use this product are limited to research and clinical diagnostics, and expressly exclude the right to use in human therapeutics, vaccines, and any use involving the administration of this product to humans.

 
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