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 >  Protein>TNFSF11 >RAL-H52A6

Human TNFSF11 / RANKL / CD254 Protein, Mouse IgG2a Fc Tag

分子别名(Synonym)

RANKL,CD254,TRANCE,OPGL,ODF

表达区间及表达系统(Source)

Human TNFSF11, Mouse IgG2a Fc Tag (RAL-H52A6) is expressed from human 293 cells (HEK293). It contains AA Gly 64 - Asp 245 (Accession # AAC51762.1 ).

Predicted N-terminus: Glu

Request for sequence

蛋白结构(Molecular Characterization)

TNFSF11 Structure

This protein carries a mouse IgG2a Fc tag at the N-terminus

The protein has a calculated MW of 47.4 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

TNFSF11 SDS-PAGE

Human TNFSF11, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

TNFSF11 ELISA

Immobilized Human Osteoprotegerin, His Tag (Cat. No. TNB-H5220) at 2 μg/mL (100 μL/well) can bind Human TNFSF11, Mouse IgG2a Fc Tag (Cat. No. RAL-H52A6) with a linear range of 0.2-2 ng/mL (QC tested).

Protocol

TNFSF11 ELISA

Immobilized Human TNFSF11, Mouse IgG2a Fc Tag (Cat. No. RAL-H52A6) at 2 μg/mL (100 μL/well) can bind Anti-Human TNFSF11 Antibody, Human IgG1 with a linear range of 0.2-2 ng/mL (Routinely tested).

Protocol

 

背景(Background)

Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), TNF-related activation-induced cytokine (TRANCE), osteoprotegerin ligand (OPGL), and osteoclast differentiation factor (ODF), is known as a type II membrane protein and is a member of the tumor necrosis factor (TNF) superfamily. RANKL, through its ability to stimulate osteoclast formation and activity, is a critical mediator of bone resorption and overall bone density. Some findings also suggestion some cancer cells, particularly prostate cancer cells, can activate an increase in bone remodeling and ultimately increase overall bone production.[17] This increase in bone remodeling and bone production increases the overall growth of bone metastasizes. The overall control of bone remodeling is regulated by the binding of RANKL with its receptor or its decoy receptor, respectively, RANK and OPG.

 

前沿进展

 
 
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TNFSF11靶点信息
英文全称:Tumor necrosis factor ligand superfamily member 11
中文全称:肿瘤坏死因子配体超家族成员11
种类:Homo sapiens
上市药物数量:6详情
临床药物数量:27详情
最高研发阶段:批准上市
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