膜杰作
Star Staining
分子别名（Synonym）
RPL,Protein L
表达区间及表达系统（Source）
Biotinylated Recombinant Protein L, His,Avitag, also known as Recombinant Peptostreptococcus magnus Protein L, was expressed in E. coli at ACRObiosystems.
Predicted N-terminus: Met
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 43.7 kDa. The protein migrates as 50 kDa under reducing (R) condition (SDS-PAGE). This protein can bind to VL-Kappa.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Recombinant Protein L, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Recombinant Protein L, His,Avitag (Cat. No. RPL-P81Q7) is more than 90% and the molecular weight of this protein is around 40-56 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Mouse Human chimeric Anti-BCMA mAb (Aa01, Mouse IgG1) at 2 μg/mL (100 μL/well) can bind Biotinylated Recombinant Protein L, His,Avitag (Cat. No. RPL-P81Q7) with a linear range of 0.2-2 ng/mL (QC tested).
Protocol
Immobilized Ipilimumab at 2 μg/mL (100 μL/well) can bind Biotinylated Recombinant Protein L, His,Avitag (Cat. No. RPL-P81Q7) with a linear range of 0.2-2 ng/mL (Routinely tested).
Protocol
Immobilized nivolumab at 2 μg/mL (100 μL/well) can bind Biotinylated Recombinant Protein L, His,Avitag (Cat. No. RPL-P81Q7) with a linear range of 0.2-2 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Biotinylated Recombinant Protein L, His,Avitag (Cat. No. RPL-P81Q7) on SA Biosensor, can bind Monoclonal Anti-Human CD19 Antibody, Mouse IgG2a with an affinity constant of 13.6 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Biotinylated Recombinant Protein L, His,Avitag (Cat. No. RPL-P81Q7) on SA Biosensor, can bind Anti-CD19 (FMC63), scFv, His Tag with an affinity constant of 74.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Biotinylated Recombinant Protein L, His,Avitag (Cat. No. RPL-P81Q7) on SA Biosensor, can bind Anti-BCMA scFv,His Tag with an affinity constant of 6.56 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Biotinylated Recombinant Protein L, His,Avitag (Cat. No. RPL-P81Q7) on SA Biosensor, can bind Anti-Human BCMA MAb (human IgG1) with an affinity constant of 14 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
活性（Bioactivity）-FACS
2e5 of anti-BCMA CAR-293 cells were stained with 100 μL of 10 μg/mL of Biotinylated Recombinant Protein L, His,Avitag (Cat. No. RPL-P81Q7) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (QC tested).
Protocol
2e5 of anti-CD19 CAR-293 cells were stained with 100 μL of 10 μg/mL of Biotinylated Recombinant Protein L, His,Avitag (Cat. No. RPL-P81Q7) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).
Protocol
背景（Background）
Protein L was isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind Ig(IgG,IgM,IgA,IgE and IgD) through L chain interaction, from which the name was suggested. Despite this wide-ranging binding capability with respect to Ig classes, Protein L is not a universal immunoglobilin-binding protein. Binding of Protein L to immunoglobulins is restricted to those containing kappa light chains (i.e., k chain of the VL domain). In humans and mice, kappa (k) light chains predominate. The remaining immunoglobulins have lambda (l) light chains. The recombinant protein contains four immunoglobulin (Ig) binding domains (Bdomains) of the native protein. Besides antibody, protein L is also suitable for binding of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).
