登录 | 注册    关注公众号  
微信公众号
搜索
 >  其他蛋白>CD72 >CD2-HF258

FITC-Labeled Human CD72 Protein, Fc Tag

分子别名(Synonym)

CD72,Lyb-2,Lyb2

表达区间及表达系统(Source)

FITC-Labeled Human CD72, Fc Tag (CD2-HF258) is expressed from human 293 cells (HEK293). It contains AA Arg 117 - Asp 359 (Accession # P21854-1). It is the FITC labeled form of Human CD72, Fc Tag (Cat. No.CD2-H5251).

Predicted N-terminus: Arg 117

Request for sequence

蛋白结构(Molecular Characterization)

Online(Arg 117 - Asp 359) P21854-1

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 54.2 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Conjugate

FITC

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 535 nm

标记(Labeling)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.

蛋白标记度(FITC:Protein Ratio)

The FITC to protein molar ratio is 2-5.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

FITC-Labeled Human CD72, Fc Tag (Cat. No. CD2-HF258) SDS-PAGE gel

FITC-Labeled Human CD72, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

背景(Background)

B-cell differentiation antigen CD72 is also known as Lyb-2. CD72 contains one C-type lectin domain. CD72 is a protein active in the immune system of animals. CD72 consists of two identical halves, each of about 39-43 kD, and is a C-type lectin. Its primarily locus of expression is B-cells, where it appears to mediate aspects of B-cell - T-cell interaction. CD72 plays a role in B-cell proliferation and differentiation. CD72 is a ligand for CD5.

 

前沿进展

 
 
货号/价格
文档
联系电话:
400-682-2521(全国)
010-53681107(北京)
021-31168505(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定