登录 | 注册    关注公众号  
微信公众号
搜索
 >  经MALS验证的蛋白>IL-17 RA & IL-17 RC >ILC-H5257

Human IL-17 RA&IL-17 RC Heterodimer Protein, Fc Tag&Fc Tag (MALS verified)

分子别名(Synonym)

IL-17 RA & IL-17 RC,IL-17RA & IL-17RC,CD217 & IL-17 RC

表达区间及表达系统(Source)

Human IL17RA&IL17RC Protein, Fc Tag&Fc Tag (ILC-H5257) is expressed from human 293 cells (HEK293). It contains AA Leu 33 - Trp 320 (IL-17RA) & Leu 21 - His 465 (IL-17RC) (Accession # Q96F46-1 (IL-17RA) & NP_703190.1 (IL-17RC)).

Predicted N-terminus: Leu 33 (IL-17RA) & Leu 21 (IL-17RC)

Request for sequence

蛋白结构(Molecular Characterization)

IL-17 RA & IL-17 RC Structure

Human IL17RA&IL17RC Protein, Fc Tag&Fc Tag is produced by co-expression of IL-17RA and IL-17RC, has a calculated MW of 60.0 kDa (IL-17RA) & 75.7 kDa (IL-17RC). Subunit IL-17RA is fused with a human IgG1 Fc tag at the C-terminus and subunit IL-17RC is fused with a human IgG1 Fc tag at the C-terminus. The reducing (R) heterodimer protein migrates as 80-90 kDa and 100-110 kDa due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20 to -70°C for 12 months in lyophilized state from date of receipt;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

IL-17 RA & IL-17 RC SDS-PAGE

Human IL17RA&IL17RC Protein, Fc Tag&Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

IL-17 RA & IL-17 RC SEC-MALS

The purity of Human IL17RA&IL17RC Protein, Fc Tag&Fc Tag(Cat. No. ILC-H5257) is more than 90% and the molecular weight of this protein is around 180-210 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

IL-17 RA & IL-17 RC ELISA

Immobilized Human IL-17A&IL-17F Heterodimer Protein, His Tag&Strep II Tag at 2 μg/mL (100 μL/well) can bind Human IL17RA&IL17RC Protein, Fc Tag&Fc Tag (Cat. No. ILC-H5257) with a linear range of 1-20 ng/mL (QC tested).

Protocol

IL-17 RA & IL-17 RC ELISA

Immobilized Human IL17A, Tag Free at 2 μg/mL (100 μL/well) can bind Human IL17RA&IL17RC Protein, Fc Tag&Fc Tag (Cat. No. ILC-H5257) with a linear range of 0.6-10 ng/mL (Routinely tested).

Protocol

IL-17 RA & IL-17 RC ELISA

Immobilized Human IL-17F (H161R), His Tag (Cat. No. ILF-H4240) at 2 μg/mL (100 μL/well) can bind Human IL17RA&IL17RC Protein, Fc Tag&Fc Tag (Cat. No. ILC-H5257) with a linear range of 1-39 ng/mL (Routinely tested).

Protocol

 

活性(Bioactivity)-SPR

IL-17 RA & IL-17 RC SPR

Human IL17RA&IL17RC Protein, Fc Tag&Fc Tag (Cat. No. ILC-H5257) captured on CM5 chip via anti-human IgG Fc antibody can bind Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag (Cat. No. ILF-H52W6) with an affinity constant of 50.8 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

IL-17 RA & IL-17 RC BLI

Loaded Human IL17RA&IL17RC Protein, Fc Tag&Fc Tag (Cat. No. ILC-H5257) on Protein A Biosensor, can bind Human IL17A, Tag Free with an affinity constant of 0.13 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

背景(Background)

Also binds heterodimers formed by IL17A and IL17F as part of a heterodimeric complex with IL17RC. Cytokine binding triggers homotypic interaction of IL17RA and IL17RC chains with TRAF3IP2 adapter, leading to TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways, ultimately resulting in transcriptional activation of cytokines, chemokines, antimicrobial peptides and matrix metalloproteinases, with potential strong immune inflammation.

 

 
货号/价格
文档
联系电话:
400-682-2521(全国)
010-53681107(北京)
021-31168505(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
IL-17 RA & IL-17 RC靶点信息
英文全称:Interleukin-17 receptor A
中文全称:白细胞介素-17受体A亚基
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:1详情
最高研发阶段:批准上市
查看更多信息
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定