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Magnetic Beads™ Streptavidin

ItemsSize (2mg)Size(5mg X 2)
Particle size2 μm2 μm
Physical appearancePowder mixturePowder mixture
Amount of Coupled Protein>1000 pmol/mg Beads>1000 pmol/mg Beads
Binding Capacity>300 pmol (45 μg) antibody/mg Beads>300 pmol (45 μg) antibody/mg Beads
FormulationPBS, pH7.4, with 10% TrehalosePBS, pH7.4, with 10% Trehalose
Reconstitution5 mL ultrapure water (1 mg beads/mL)5 mL ultrapure water (1 mg beads/mL)

Background/背景

The streptavidin magnetic beads are uniform size, narrow size distribution with large surface area and unique surface coating, it is easy to capture the biotinylated proteins or other molecules, and the bounded protein have no activity lost, can help us get the best performance and highly reproducible results.

Application/应用

This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.

Reconstitution/重构方法

See Certificate of Analysis (CoA) for detailed instruction.

Storage/存储

This product is stable for 1 year when stored at -20 ℃.

Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.

Assay Principles/原理

0

Antibody Purification:

1. Reconstitute the beads with ultrapure water to 1 mg/mL, and wash the beads for three times.

2. Dilute the biotinalyted protein or antibodies with Assay Buffer and add to the beads. Incubate for 60 minutes at room temperature in a rotary mixer.

3. Wash the beads for four times, and add Assay Buffer to the beads. The beads are ready to use for the next experiment.

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