|Items||Size （5mg）||Size（5mg X 2）|
|Particle size||2 μm||2 μm|
|Amount of D-Biotin coupled||>1000 pmol / mg Beads||>1000 pmol / mg Beads|
|Capacity||>300 pmol (20 μg) antibody / mg Beads||>300 pmol (20 μg) antibody / mg Beads|
|Formulation||PBS, 0.1% (w/v) BSA, 0.1% (v/v) proclin-300, pH7.4, with 10% trehalose||PBS, 0.1% (w/v) BSA, 0.1% (v/v) proclin-300, pH7.4, with 10% trehalose|
|Product Format||>300 pmol / mg Beads||>300 pmol / mg Beads|
|Reconstitution||Reconstitute 5 mg / bottle Beads with 5 mL ultrapure water (1 mg beads / mL)||Reconstitute 5 mg / bottle Beads with 5 mL ultrapure water (1 mg beads / mL)|
The streptavidin magnetic beads are uniform size, narrow size distribution with large surface area and unique surface coating, it is easy to capture the biotinylated proteins or other molecules, and the bounded protein have no activity lost, can help us get the best performance and highly reproducible results.
This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
See Certificate of Analysis for details of reconstitution instruction and specific concentration.
Upon receipt, please store the beads at -20℃ in a power state.
Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.
Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 mol/L, the Biotinylated protein can bind to the SA beads irreversibly. The Magenetic beads are pre-coupled with Streptavidin tetramer protein and blocked with BSA, it is a ready-to-use beads, which can be used for binding biotinylated proteins and antibodies.
a) Reconstitute the beads with ultrapure water to 1mg/mL, and wash the beads for three times.
b) Dilute the biotinalytated protein or antibodies with Assay Buffer, and add to the beads, incubate for 60 minutes at room temperature in a rotary mixer.
c) Wash the beads for four times, and add assay buffer to beads for next experiment.