Recombinant Proteins iso9001 ISO13485
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SARS-CoV-2 Nucleocapsid Protein Titer Assay Kit

For research use only.

IDComponentsSize
RAS010-C01Anti-SARS-CoV-2 Nucleocapsid Protein Antibody60μg
RAS010-C02SARS-CoV-2 Nucleocapsid Protein10μg
RAS010-C03Biotin-Anti-SARS-CoV-2 Nucleocapsid Protein Antibody10μg
RAS010-C04Streptavidin-HRP10μg
RAS010-C05High-bind Plate1 plate
RAS010-C06Coating Buffer12 mL
RAS010-C0710xWashing Buffer 50 mL
RAS010-C08Blocking / Dilution Buffer50 mL
RAS010-C09Substrate Solution12 mL

Background/背景

Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.

Application/应用

This kit is developed for detecting the SARS-CoV-2 Nucleocapsid Protein in cell supernatant or SARS-CoV-2 virus in vitro.

Reconstitution/重构方法

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage/存储

Upon receipt, please store the lyophilized products at -20℃. After reconstitution, the stock solution should be kept at -70℃. Upon receipt, please store the plate at Room Temperature, and please store the buffer at -20℃.

It is recommended not to freeze thaw more than 3 times.

This product is stable under storage conditions: 12 months in sealed state;
Used within 3 months after opening.

Assay Principles/原理

This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of SARS-CoV-2 Nucleocapsid Protein. The kit consists of Anti-SARS-CoV-2 Nucleocapsid Antibody, an SARS-CoV-2 Nucleocapsid Protein as Control, an biotin-Anti-SARS-CoV-2 Nucleocapsid Antibody and HRP-Streptavidin.

Your experiment will include 6 simple steps:

a) Coat the plate with Anti-SARS-CoV-2 Nucleocapsid Antibody.

b) After washing and blocking, add your sample to the plate, take the SARS-CoV-2 Nucleocapsid protein as Control sample. The samples and Control sample are diluted by Lysis Buffer.

c) Add a diluted Secondary antibody biotin-anti-SARS-CoV-2 Nucleocapsid Antibody to the plate. The Secondary antody is diluted by Dilution Buffer.

d) Add a diluted Streptavidin-HRP to the plate.

e) Wash the plate and add TMB or other colorimetric HRP substrate.

f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

 

Typical Data/典型数据

Biotinylated Human ELISA

Detection SARS-CoV-2 Nucleocapsid Protein by ELISA Assay.
Immobilized Anti-SARS-CoV-2 Nucleocapsid Antibody at 4 μg/mL (100 μL/well) can bind SARS-CoV-2 Nucleocapsid Protein. And then add Biotinylated Anti-SARS-CoV-2 Nucleocapsid Antibody at 1:5000. Detection was performed using high sensitivity HRP-conjugated streptavidin with sensitivity of 12.5 pg/mL (QC tested).

Protocol

前沿进展

 
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