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SARS-CoV-2 Nucleocapsid Protein Titer Assay Kit

For research use only.

IDComponentsSize
RAS010-C01Pre-coated Anti-SARS-CoV-2 Nucleocapsid Antibody Microplate1 plate
RAS010-C02SARS-CoV-2 Nucleocapsid Protein10 ug
RAS010-C03Biotin-Anti-SARS-CoV-2 Nucleocapsid Antibody100 μL
RAS010-C04Streptavidin-HRP100 μL
RAS010-C0510xWashing Buffer 50 mL
RAS010-C06Dilution Buffer50 mL
RAS010-C07Substrate Solution12 mL
RAS010-C08Stop Solution7 mL

Background/背景

Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.

Application/应用

This kit is developed for detecting the SARS-CoV-2 Nucleocapsid Protein in cell supernatant or SARS-CoV-2 virus in vitro.

It is for research use only.

Reconstitution/重构方法

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage/存储

The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

The opened kit should be stored per components table.The shelf life is 30 days from the date of opening.

Assay Principles/原理

This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of SARS-CoV-2 Nucleocapsid Protein. The kit consists of Anti-SARS-CoV-2 Nucleocapsid Antibody, an SARS-CoV-2 Nucleocapsid Protein as Control, an biotin-Anti-SARS-CoV-2 Nucleocapsid Antibody and HRP-Streptavidin.

Your experiment will include 5 simple steps:

a) Add your sample to the plate, take the SARS-CoV-2 Nucleocapsid protein as Control sample. The samples and Control sample are diluted by Lysis Buffer.

b) Add a diluted Secondary antibody biotin-anti-SARS-CoV-2 Nucleocapsid Antibody to the plate. The Secondary antody is diluted by Dilution Buffer.

c) Add a diluted Streptavidin-HRP to the plate.

d) Wash the plate and add TMB or other colorimetric HRP substrate.

e) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

 

Typical Data/典型数据

Biotinylated Human ELISA

Detection of Monoclonal SARS-CoV-2 Nucleocapsid Protein titer by sandwich-ELISA Assay.
Immobilized Anti-SARS-CoV-2 Nucleocapsid Protein Antibody at 4 μg/mL (100 μL/well) can bind SARS-CoV-2 Nucleocapsid Protein and Biotin-Anti-SARS-CoV-2 Nucleocapsid Antibody. Detection was performed using Streptavidin-HRP with sensitivity of 12.5 pg/mL (QC tested).

Protocol

前沿进展

 
 
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