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Biotinylated Human FAP Protein, His,Avitag™, active dimer (MALS verified)

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利用生物素-链霉亲和素系统的高特异性和高灵敏度的结合特性,可将生物素化蛋白与链霉亲和素相关产品联合应用于各类实验,与非生物素体系相比,检测速度更快、结合能力更强、非特异背景更低。

分子别名(Synonym)

FAP,FAPalpha,SIMP,Seprase,APCE

表达区间及表达系统(Source)

Biotinylated Human FAP Protein, His,Avitag (FAP-H82Q6) is expressed from human 293 cells (HEK293). It contains AA Leu 26 - Asp 760 (Accession # Q12884-1).

Predicted N-terminus: His

Request for sequence

蛋白结构(Molecular Characterization)

Online(Leu 26 - Asp 760) Q12884-1

This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 93.1 kDa. The protein migrates as 100-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human FAP Protein, His,Avitag (Cat. No. FAP-H82Q6) SDS-PAGE gel

Biotinylated Human FAP Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Biotinylated Human FAP Protein, His,Avitag (Cat. No. FAP-H82Q6) MALS images

The purity of Biotinylated Human FAP Protein, His,Avitag (Cat. No. FAP-H82Q6) is more than 90% and the molecular weight of this protein is around 200-230 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Biotinylated Human FAP Protein, His,Avitag (Cat. No. FAP-H82Q6) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Sibrotuzumab with a linear range of 0.1-1 ng/mL (QC tested).

Protocol

 

活性(Bioactivity)

Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >4000 pmol/min/µg (QC tested).

Protocol

 

活性(Bioactivity)-FACS

Biotinylated Human FACS

2e5 of Anti-FAP CAR-293 cells were stained with 100 μL of 10 μg/mL of Biotinylated Human FAP Protein, His,Avitag (Cat. No. FAP-H82Q6) and negative control protein respectively , washed and then followed by PE-SA and analyzed with FACS (Routinely tested).

Protocol

 

背景(Background)

FAP (also known as seprase) is a Type II transmembrane serine protease. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences. Degrade also gelatin, heat-denatured type I collagen. Also has dipeptidyl peptidase activity, with a preference for Ala-Pro, Ile-Pro, Gly-Pro, Arg-Pro and Pro-Pro. The plasma membrane form, in association with either DPP4, PLAUR or integrins, is involved in the pericellular proteolysis of the extracellular matrix (ECM), and hence promotes cell adhesion, migration and invasion through the ECM. Promotes glioma cell invasion through the brain parenchyma by degrading the proteoglycan brevican. Acts as a tumor suppressor in melanocytic cells through regulation of cell proliferation and survival in a serine protease activity-independent manner.

 

 

前沿进展

 
 
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FAP靶点信息
英文全称:Fibroblast activation protein alpha
中文全称:成纤维细胞激活蛋白α
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:27详情
最高研发阶段:临床二期
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