登录 | 注册    关注公众号  
微信公众号
搜索
 >  Protein>TGF-beta RII >TG2-H82E4

Biotinylated Human TGF-beta RII / TGFBR2 Protein, His,Avitag™

热销产品推荐:

ACROBiosystems提供针对多种生物标志物靶标的lHC抗体产品,具有:验证充分、质量可靠、灵活授权及免疫组化检测服务支持!

分子别名(Synonym)

TGFBR2,TGFR2,TbetaR-II,TGFβR2

表达区间及表达系统(Source)

Biotinylated Human TGF-beta RII, His,Avitag (TG2-H82E4) is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Asp 159 (Accession # P37173-1).

Predicted N-terminus: Thr 23

Request for sequence

蛋白结构(Molecular Characterization)

Online(Thr 23 - Asp 159) P37173-1

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 19.1 kDa. The protein migrates as 28-40 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human TGF-beta RII, His,Avitag (Cat. No. TG2-H82E4) SDS-PAGE gel

Biotinylated Human TGF-beta RII, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human Latent TGFB1, His Tag (Cat. No. TG1-H524x) at 1 μg/mL (100 μL/well) can bind Biotinylated Human TGF-beta RII, His,Avitag (Cat. No. TG2-H82E4) with a linear range of 5-78 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Serial dilutions of Human TGF-beta RII, His Tag (Cat. No. TG2-H52H5) were added into Human Latent TGFB1, His Tag (Cat. No. TG1-H524x) : Biotinylated Human TGF-beta RII, His,Avitag (Cat. No. TG2-H82E4) binding reactions. The half maximal inhibitory concentration (IC50) is 0.39287 μg/mL (Routinely tested).

Protocol

背景(Background)

TGF-beta receptor type-2 (TGFBR2 or TGFR-2) is also known as TGF-beta type II receptor, Transforming growth factor-beta receptor type II, TbetaR-II, TGFβR2, which is a homodimer or heterohexamer, belongs to the protein kinase superfamily, TKL Ser/Thr protein kinase family and TGFB receptor subfamily. TGFR2 / TGFBR2 binds TGF-β1 / TGFB1 and TGF-β3 / TGFB3 with high affinity and TGF-β2 / TGFB2 with a much lower affinity. This type I I receptor forms a heterodimeric complex with type I receptor and is essential for signal transduction. Upon ligand binding, the TGFR2 autophosphorylates its cytoplasmic domain and subsequently phosphorylates the downstream molecules which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation.

 

 

前沿进展

 
 
货号/价格
产品推荐
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
TGF-beta RII靶点信息
英文全称:Transforming growth factor beta receptor 2
中文全称:转化生长因子β受体2
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:4详情
最高研发阶段:临床二期
查看更多信息
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定