膜杰作
Star Staining
分子别名（Synonym）
CD93,CDw93,C1QR1,MXRA4,C1qR,C1qR(P),C1qRP,ECSM3
表达区间及表达系统（Source）
Human C1q R1, His Tag (C11-H5228) is expressed from human 293 cells (HEK293). It contains AA Thr 22 - Lys 580 (Accession # AAH28075).
Predicted N-terminus: Thr 22
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 59.2 kDa. The protein migrates as 70-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human C1q R1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Human C1q R1, His Tag (Cat. No. C11-H5228) is more than 90% and the molecular weight of this protein is around 75-85 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human C1q R1, His Tag (Cat. No. C11-H5228) at 5 μg/mL (100 μL/well) can bind Human IGFBP-7, Fc Tag (Cat. No. IG7-H5259) with a linear range of 0.6-20 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Human IGFBP-7, His Tag (Cat. No. IG7-H5240) immobilized on CM5 Chip can bind Human C1q R1, His Tag (Cat. No. C11-H5228) with an affinity constant of 16.7 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
背景（Background）
CD antigen CD93 is also known as Complement component C1q receptor (C1QR1). which belongs to the Group XIV C-Type lectin family, a group containing two other members, endosialin (CD248) and thrombomodulin. CD93 contains one C-type lectin domain and five EGF-like domains. CD93 is highly expressed in endothelial cells, platelets, cells of myeloid origin, such as monocytes and neutrophils. CD93 is receptor for C1q, mannose-binding lectin (MBL2) and pulmonary surfactant protein A (SPA). CD93 may mediate the enhancement of phagocytosis in monocytes and macrophages upon interaction with soluble defense collagens and may play a role in intercellular adhesion.
