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 >  经MALS验证的蛋白>APRIL >APL-H52D1

Human APRIL / TNFSF13 Protein, His,Flag Tag, active trimer (MALS verified)

分子别名(Synonym)

APRIL,TNFSF13,TALL-2,TRDL-1,CD256,TALL2,ZTNF2

表达区间及表达系统(Source)

Human APRIL Protein, His,Flag Tag (APL-H52D1) is expressed from human 293 cells (HEK293). It contains AA Gln 111 - Leu 250 (Accession # O75888-1 ) trimer Design.

Predicted N-terminus: His

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag

The protein has a calculated MW of 50.2 kDa. The protein migrates as 50-55 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

APRIL SDS-PAGE

Human APRIL Protein, His,Flag Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

APRIL SEC-MALS

The purity of Human APRIL Protein, His,Flag Tag (Cat. No. APL-H52D1) is more than 90% and the molecular weight of this protein is around 55-80 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

APRIL ELISA

Immobilized Human BCMA, Fc Tag (Cat. No. BC7-H5254) at 2 μg/mL (100 μL/well) can bind Human APRIL Protein, His,Flag Tag (Cat. No. APL-H52D1) with a linear range of 10-39 ng/mL (QC tested).

Protocol

APRIL ELISA

Immobilized Human APRIL Protein, His,Flag Tag (Cat. No. APL-H52D1) at 1 μg/mL (100 μL/well) can bind Human TACI Protein, Fc Tag (Cat. No. TAI-H5256) with a linear range of 0.8-25 ng/mL (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

APRIL BLI

Loaded Human APRIL Protein, His,Flag Tag (Cat. No. APL-H52D1) on HIS1K Biosensor, can bind Human BCMA, Fc Tag (Cat. No. BC7-H5254) with an affinity constant of 1.88 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

APRIL BLI

Loaded Human APRIL Protein, His,Flag Tag (Cat. No. APL-H52D1) on HIS1K Biosensor, can bind Human TACI Protein, Fc Tag (Cat. No. TAI-H5256) with an affinity constant of 75 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

背景(Background)

APRIL(a proliferation-inducing ligand) is also known as Tumor necrosis factor ligand superfamily member 13, TALL-2, TRDL-1, CD256, TNFFSF 13, cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. APRIL is a cytokine of the tumor necrosis factor family associated mainly with hematologic malignancies. The closely related TNF family ligands B cell activation factor (BAFF) and a proliferation-inducing ligand (APRIL) serve in the generation and maintenance of mature B-lymphocytes. Both BAFF and APRIL assemble as homotrimers that bind and activate several receptors that they partially share. BAFF-APRIL heteromers of different stoichiometries have distinct receptor-binding properties and activities. In addition, expression of APRIL was regulated by miR-145 in GC cells.

 

 
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APRIL靶点信息
英文全称:Tumor necrosis factor ligand superfamily member 13
中文全称:肿瘤坏死因子配体超家族成员13
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:10详情
最高研发阶段:批准上市
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