|Plate Blocking:||2% BSA Blocking Buffer|
|Formulations||Clear, 96-well plates, coated with 100μL of Streptavidin tetramer, blocked with 300μL of 2% BSA Blocking Buffer and captured 0.1 μg/well of biotinylated SARS-CoV2 Spike S1 protein.|
The newly identified 2019 novel coronavirus (SARS-CoV-2) has posed a serious threat to human health, and it spreads quickly. A rapid and effective Assay kit can help us find the COVID-19 effectively as early as possible, and detect the levels of anti-SARS-CoV-2 antibodies or the target ACE-2 protein in serum.
This SARS-CoV2 Spike S1 protein- Coated Plate is intended for ELISA Assay and Biopannning.
- Flexible experimental design because of the plate consist with detachable 8-well strips.
- Save your experiment time by pre-coated and blocked.
- No denaturing of the protein component of a conjugate upon binding.
- Ideal for binding anti-SARS2-CoV-2 antibodies or ACE2 protein that typically exhibit poor binding to polystyrene.
Upon receipt store plates at 4°C in unopened pouches. Once opened, place unused plates in a resealable bag with desiccant and store at 4°C. Plates are shipped at 4°C.
Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 mol/L, the Biotinylated molecules can bind to the SA irreversibly. The SARS-CoV2 Spike S1 protein-Coated Plates are immobilized with biotinylated SARS-CoV2 Spike S1 protein to a Streptavidin tetramer protein coated plate, which is easy to use and widely available for applications.
Example ELISA Procedure.
Your experiment will include 5 simple steps:
a) Blocking the plate and wash the plate.
b) Add your sample to the plate, take the Anti-SARS-CoV-2 antibody or ACE-2 protein as Control sample. Incubation and wash the plate.
c) Add a diluted Secondary antibody HRP-Goat anti-Human IgG, Fc to the plate and incubation.
d) Wash the plate and next add TMB or other colorimetric HRP substrate.
e) Stop the substrate reaction by add diluted acid, and Read OD at 450 nm, the OD Value reflects the amount of antibody bound.
Example Biopanning Procedure
Immobilized biotinylated SARS-CoV2 Spike S1 protein at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind anti-SARS-CoV-2 S1 antibody with a linear range of 0.1-3 ng/mL (QC tested).
Immobilized biotinylated SARS-CoV2 Spike S1 protein at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind ACE2 with a linear range of 0.2-4 ng/mL (QC tested).