登录 | 注册    关注公众号  
微信公众号
搜索
 >  其他蛋白>Streptavidin >SP-11

Streptavidin Coated Plates, Clear, 12×8-Well Strips, White Frame

产品展示(Product Show)

Streptavidin Plate

背景(Background)

Biotin avidin system (BAS) is a new bioreaction amplification system developed in the late 1970s. With the advent of various biotin derivatives, BAS was soon widely used in various fields of medicine. A large number of studies have confirmed in recent years that the biotin avidin system can be combined with almost kind of markers for research success. The strong binding of biotin and avidin with high affinity and multi-stage amplification effect make BAS immunolabeling and related tracer analysis more sensitive. It has become a new technique widely used in the qualitative and quantitative detection of trace antigens and antibodies, as well as in localization observation research.

应用说明(Application)

This Streptavidin Coated Plate is intended for Immunoassay and ELISA. The plate could be seperated to 12× 8-well strips and the frame is white.

存储(Storage)

The unopened plate should be stored at 2°C to 8°C,The expiry date of the plate is 12 months. Once opened, place unused plates in a resealable bag with desiccant and store at 2°C to 8°C, The shelf life is 1 month from the date of opening.

原理(Assay Principles)

Example ELISA Procedure.

Your experiment will include 5 simple steps:

a) Add your Biotinylated sample to the plate, incubation for 1 hour and wash the plate.

b) Add your sample to the plate, incubation for 1 hour and wash the plate.

c) Add a diluted Detection antibody to the plate and incubation.

d) Wash the plate and next add TMB or other colorimetric HRP substrate.

e) Stop the substrate reaction by add diluted acid, and Read OD at 450 nm, the OD Value reflects the amount of antibody bound.

Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10−14 mol/L, the Biotinylated molecules can bind to the SA irreversibly. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions. The Streptavidin Coated Plates we provide are easy to use and widely available for applications.

 

典型数据-Typical Data

Streptavidin ELISA

Immobilized Biotinylated Human CD19 (20-291), His,Avitag (Cat. No. CD9-H82E9) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 12×8-Well Strips, White Frame (Cat. No. SP-11), can bind Anti-FMC63 antibody with a linear range of 0.1-3 ng/mL (QC tested).

Protocol

Streptavidin ELISA

Immobilized Biotinylated Human PD-1, Fc,Avitag,His Tag (Cat. No. PD1-H82F4) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 12×8-Well Strips, White Frame (Cat. No. SP-11), can bind Human PD-L1, mFc Tag with a linear range of 0.4-6.25 ng/mL (QC tested).

Protocol

Streptavidin ELISA

Immobilized Biotinylated Human CD20 Full Length, His,Avitag (HEK293) (Cat. No. CD0-H82E5) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 12×8-Well Strips, White Frame (Cat. No. SP-11), can bind Rituximab with a linear range of 78-625 ng/mL (QC tested).

Protocol

Streptavidin ELISA

Immobilized Biotinylated Human FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-H82W7) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 12×8-Well Strips, White Frame (Cat. No. SP-11), can bind Ipilimumab with a linear range of 78-1250 ng/mL (QC tested).

Protocol

Streptavidin ELISA

Routine stability test. After placing the Streptavidin Coated Plates, Clear, 12×8-Well Strips, White Frame (Cat.No. SP-11) at 2-8°C for 4 months, binding activity between the SP-11,the Spike RBD Protein and the Anti-SARS-CoV-2 Spike RBD Antibody showed no difference from the Plate Coated Before the test.

Protocol

Streptavidin ELISA

Cat. No. SP-11, Adsorption stability test of avidin protein. After three SA plates were washed with buffer solution for three times, five times and ten times respectively, binding activity between the SA plate, the Spike RBD Protein and the Anti-SARS-CoV-2 Spike RBD Antibody showed no difference. The test shows that the SA protein has good stability on the plate.

Protocol

前沿进展

 
 
货号/价格
文档
联系电话:
400-682-2521(全国)
010-53681107(北京)
021-31168505(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
前沿进展
点击查看详细
相关产品
View All Streptavidin

消息提示

请输入您的联系方式,再点击提交!

确定