|Plate Blocking||2% BSA Blocking Buffer|
|Formulations||Clear, 96-well plates, coated with 100uL of streptavidin tetramer and blocked with 200uL of 2% BSA Blocking Buffer|
|CV% of plates/wells||< 10%|
|Type||Detection Plate, Immunoassay, ELISA|
Biotin avidin system (BAS) is a new bioreaction amplification system developed in the late 1970s. With the advent of various biotin derivatives, BAS was soon widely used in various fields of medicine. A large number of studies have confirmed in recent years that the biotin avidin system can be combined with almost kind of markers for research success. The strong binding of biotin and avidin with high affinity and multi-stage amplification effect make BAS immunolabeling and related tracer analysis more sensitive. It has become a new technique widely used in the qualitative and quantitative detection of trace antigens and antibodies, as well as in localization observation research.
This Streptavidin Coated Plate is intended for Immunoassay and ELISA. The plate could be seperated to 12× 8-well strips and the frame is white.
The unopened plate should be stored at 2°C to 8°C,The expiry date of the plate is 12 months. Once opened, place unused plates in a resealable bag with desiccant and store at 2°C to 8°C, The shelf life is 1 month from the date of opening.
Example ELISA Procedure.
Your experiment will include 5 simple steps:
a) Add your Biotinylated sample to the plate, incubation for 1 hour and wash the plate.
b) Add your sample to the plate, incubation for 1 hour and wash the plate.
c) Add a diluted Detection antibody to the plate and incubation.
d) Wash the plate and next add TMB or other colorimetric HRP substrate.
e) Stop the substrate reaction by add diluted acid, and Read OD at 450 nm, the OD Value reflects the amount of antibody bound.
Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10−14 mol/L, the Biotinylated molecules can bind to the SA irreversibly. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions. The Streptavidin Coated Plates we provide are easy to use and widely available for applications.
Immobilized Biotinylated SARS-CoV-2 Spike RBD at 1 μg/mL(100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Anti-SARS-CoV-2 Spike RBD Antibody with a linear range of 0.0244-50 ng/mL (QC tested).
Immobilized Biotinylated Human CD19 (20-291), His,Avitag (Cat. No. CD9-H82E9) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 12×8-Well Strips, White Frame (Cat. No. SP-11), can bind Anti-FMC63 antibody with a linear range of 0.1-3 ng/mL (Routinely tested).
Immobilized Biotinylated Human PD-1, Fc,Avitag,His Tag (Cat. No. PD1-H82F4) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 12×8-Well Strips, White Frame (Cat. No. SP-11), can bind Human PD-L1, mFc Tag with a linear range of 0.4-6.25 ng/mL (Routinely tested).
Routine stability test. After placing the Streptavidin Coated Plates, Clear, 12×8-Well Strips, White Frame (Cat.No. SP-11) at 2-8°C for 4 months, binding activity between the SP-11,the Spike RBD Protein and the Anti-SARS-CoV-2 Spike RBD Antibody showed no difference from the Plate Coated Before the test.
Cat. No. SP-11, Adsorption stability test of avidin protein. After three SA plates were washed with buffer solution for three times, five times and ten times respectively, binding activity between the SA plate, the Spike RBD Protein and the Anti-SARS-CoV-2 Spike RBD Antibody showed no difference. The test shows that the SA protein has good stability on the plate.