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Streptavidin Coated Plates, Clear, 96-Well

Background/背景

Application/应用

This Streptavidin Coated Plate is intended for Immunoassay and ELISA.

Storage/存储

The unopened plate should be stored at 2°C to 8°C,The expiry date of the plate is 12 months. Once opened, place unused plates in a resealable bag with desiccant and store at 2°C to 8°C, The shelf life is 1 month from the date of opening.

Assay Principles/原理

Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10−14 mol/L, the Biotinylated molecules can bind to the SA irreversibly. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions. The Streptavidin Coated Plates we provide are easy to use and widely available for applications. Example ELISA Procedure

Your experiment will include 5 simple steps:

a) Add your Biotinylated sample to the plate, incubation for 1 hour and wash the plate.

b) Add your sample to the plate, incubation for 1 hour and wash the plate.

c) Add a diluted Detection antibody to the plate and incubation.

d) Wash the plate and next add TMB or other colorimetric HRP substrate.

e) Stop the substrate reaction by add diluted acid, and Read OD at 450 nm, the OD Value reflects the amount of antibody bound.

 

Typical Data/典型数据

Biotinylated Human ELISA

Immobilized Biotinylated Human CD19 (20-291), His,Avitag (Cat. No. CD9-H82E9) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Anti-FMC63 antibody with a linear range of 0.1-3 ng/mL (QC tested).

Protocol

Biotinylated Human SPR

Immobilized Biotinylated Human PD-1, Fc,Avitag,His Tag (Cat. No. PD1-H82F4) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Human PD-L1, mFc Tag with a linear range of 0.4-6.25 ng/mL (QC tested).

Protocol

Biotinylated Human ELISA

Immobilized Biotinylated Human CD20 Full Length, His,Avitag (HEK293) (Cat. No. CD0-H82E5) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Rituximab with a linear range of 78-625 ng/mL (QC tested).

Protocol

Biotinylated Human ELISA

Immobilized Biotinylated Human FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-H82W7) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Ipilimumab with a linear range of 78-1250 ng/mL (QC tested).

Protocol

前沿进展

 
 
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400-682-2521(全国)
010-53681107(北京)
021-31168505(上海)
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order.cn@acrobiosystems.com
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tech.cn@acrobiosystems.com
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