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Biotinylated Human GUCY2C / Guanylyl cyclase C Protein, His,Avitag™ (MALS verified)

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分子别名(Synonym)

GUCY2C,GUC2C,STAR,STA receptor,hSTAR,GC-C

表达区间及表达系统(Source)

Biotinylated Human GUCY2C, His,Avitag (GUC-H82E9) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Gln 430 (Accession # P25092-1).

Predicted N-terminus: Ser 24

Request for sequence

蛋白结构(Molecular Characterization)

Online(Ser 24 - Gln 430) P25092-1

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 49.6 kDa. The protein migrates as 65-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human GUCY2C, His,Avitag (Cat. No. GUC-H82E9) SDS-PAGE gel

Biotinylated Human GUCY2C, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Biotinylated Human GUCY2C, His,Avitag (Cat. No. GUC-H82E9) MALS images

The purity of Biotinylated Human GUCY2C, His,Avitag (Cat. No. GUC-H82E9) is more than 90% and the molecular weight of this protein is around 70-85 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Biotinylated Human GUCY2C, His,Avitag (Cat. No. GUC-H82E9) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-Human GUCY2C Antibody, Human IgG1 with a linear range of 0.2-2 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Monoclonal Anti-Human GUCY2C Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human GUCY2C, His,Avitag (Cat. No. GUC-H82E9) with a linear range of 0.2-6 ng/mL (Routinely tested).

Protocol

背景(Background)

GUCY2C (Guanylyl Cyclase C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (gc) family that signal by producing cGMP. Guanylate cyclase C (GUCY2C) and its hormones guanylin and uroguanylin have recently emerged as one paracrine axis defending intestinal mucosal integrity against mutational, chemical, and inflammatory injury. GUCY2C murine CAR-T cells recognized and killed human colorectal cancer cells endogenously expressing GUCY2C. Thus, we have identified a human GUCY2C-specific CAR-T cell therapy approach that may be developed for the treatment of GUCY2C-expressing metastatic colorectal cancer.

 

 

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GUCY2C靶点信息
英文全称:Heat-stable enterotoxin receptor
中文全称:热稳定肠毒素受体
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:4详情
最高研发阶段:批准上市
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