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 >  Protein>Siglec-3 >CD3-HF224

FITC-Labeled Human Siglec-3 / CD33 Protein, His Tag

分子别名(Synonym)

CD33,SIGLEC3,gp67

表达区间及表达系统(Source)

FITC-Labeled Human Siglec-3, His Tag (CD3-HF224) is expressed from human 293 cells (HEK293). It contains AA Asp 18 - His 259 (Accession # AAH28152.1 ). It is the FITC labeled form of Human Siglec-3, His Tag (CD3-H5226).

Predicted N-terminus: Asp 18

Request for sequence

蛋白结构(Molecular Characterization)

Siglec-3 Structure

This protein carries a polyhistidine tag at the C-terminus

The protein has a calculated MW of 27.6 kDa. The protein migrates as 40-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

偶联(Conjugate)

FITC

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 535 nm

标记(Labeling)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.

蛋白标记度(Protein Ratio)

The FITC to protein molar ratio is 1.5-3.5.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Siglec-3 SDS-PAGE

FITC-Labeled Human Siglec-3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Siglec-3 ELISA

Immobilized Human Siglec-3 Antibody at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human Siglec-3, His Tag (Cat. No. CD3-HF224) with a linear range of 0.02-0.625 μg/mL (QC tested).

Protocol

 

活性(Bioactivity)-FACS

Siglec-3 FACS

2e5 of anti-Siglec-3 CAR-293 cells were stained with 100 μL of 1 μg/mL of FITC-Labeled Human Siglec-3, His Tag (Cat. No.CD3-HF224) and negative control protein respectively, FITC signal was used to evaluate the binding activity (QC tested).

Protocol

 

背景(Background)

Myeloid cell surface antigen CD33 is also known as SIGLEC3, Siglecs (sialic acid binding Ig­like lectins) and GP67, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Human CD33 / Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-­terminal Ig-­like V­-type domain, one Ig­-like C2-­type domains, a transmembrane region and a cytoplasmic tail. CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells. In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia.

 

前沿进展

 
 
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Siglec-3靶点信息
英文全称:Myeloid cell surface antigen CD33
中文全称:髓系细胞表面抗原CD33
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:32详情
最高研发阶段:批准上市
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