膜杰作
Star Staining
ViruStop
Aneuro
ComboX
ActiveMax
GENPower
ClinMax
Star Ribbon
分子别名（Synonym）
MSLN,Mesothelin,MPF
表达区间及表达系统（Source）
Mouse Mesothelin (298-600), His Tag (MSN-M52H3) is expressed from human 293 cells (HEK293). It contains AA Asp 298 - Ser 600 (Accession # Q61468-1 ).
Predicted N-terminus: Asp 298
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 36.0 kDa. The protein migrates as 42-48 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Mouse Mesothelin (298-600), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Mouse Mesothelin (298-600), His Tag (Cat. No. MSN-M52H3) is more than 90% and the molecular weight of this protein is around 40-55 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Mouse Mesothelin (298-600), His Tag (Cat. No. MSN-M52H3) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CA125, Fc,Avitag (Cat. No. CA5-H82F4) with a linear range of 0.6-10 ng/mL (QC tested).
Protocol
活性（Bioactivity）-BLI
Loaded Monoclonal Anti-Human MSLN Antibody, Human IgG1 (Cat. No. MSN-M30) on Protein A Biosensor, can bind Mouse Mesothelin (298-600), His Tag (Cat. No. MSN-M52H3) with an affinity constant of 170 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
背景（Background）
Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.
