|TAH005-C01||Streptavidin pre-coated Plate||1plate|
|TAH005-C02||Biotinylated Her2 Protein||10ug|
|TAH005-C03||Human Anti-Her2 antibody||10ug|
|TAH005-C04||HRP-anti-human IgG, Fc antibody||10ug|
Her2 is a member of the human epidermal growth factor receptor family. In recent years, it has become an important biomarker and target of therapy for oncology patients. Although Her2 has been identified as a target of therapy for years and there are a few anti-Her2 therapeutic antibodies on the market, the heat of research regarding this target remains on. ACRO anti Her2 ELISA kit is intended for PK study as well as anti Her2 therapeutic screening.
It is for research use only.
See Certificate of Analysis for details of reconstitution instruction and specific concentration.
Upon receipt, please store the lyophilized products at -20℃. After reconstitution, the stock solution should be kept at -70℃. Upon receipt, please store the plate at Room Temperature, and please store the buffer at -20℃.
Try to avoid more than 3 freeze thaw cycles.
No activity loss is observed after storage at: Room temperature (RT) for 1 month in lyophilized state; -20℃ for 1 year in lyophilized state; -70℃ for 6 months under sterile conditions after reconstitution.
This assay kit employs a standard Indirect-ELISA format, which provides a rapid quantification of Anti-Her2 antibodies through antigen binding. The Kit includes of Streptavidin pre-coated plate, the Biotinylated Her2 protein, a human anti-Her2 antibody standard, a secondary HRP-anti-Human IgG antibody and an assay diluent.
Your experiment will only have 5 simple steps:
1. Coat the plate with Biotinylated Her2 protein. Block and wash the plate.
2. Add samples to the plate, and use human anti-Her2 antibody as control. Incubate and wash the plate.
3. Add the diluted secondary HRP-anti-human IgG, Fc antibody to the plate and incubate.
4.Wash the plate and then add TMB or other colorimetric HRP substrate.
5. Stop the substrate reaction by adding the diluted acid. Read OD at 450 nm, which reflects the amount of antibody bound.