Recombinant Proteins iso9001 ISO13485
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Anti Her2 ELISA Kit

For research use only.

IDComponentsSize
TAH005-C01Streptavidin pre-coated Plate 1plate
TAH005-C02Biotinylated Her2 Protein10ug
TAH005-C03Human Anti-Her2 antibody10ug
TAH005-C04HRP-anti-human IgG, Fc antibody10ug
TAH005-C05Diluent buffer50mL

Background/背景

Her2 is a member of the human epidermal growth factor receptor family. In recent years, it has become an important biomarker and target of therapy for oncology patients. Although Her2 has been identified as a target of therapy for years and there are a few anti-Her2 therapeutic antibodies on the market, the heat of research regarding this target remains on. ACRO anti Her2 ELISA kit is intended for PK study as well as anti Her2 therapeutic screening.

Application/应用

It is for research use only.

Reconstitution/重构方法

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage/存储

Upon receipt, please store the lyophilized products at -20℃. After reconstitution, the stock solution should be kept at -70℃. Upon receipt, please store the plate at Room Temperature, and please store the buffer at -20℃.

Try to avoid more than 3 freeze thaw cycles.

No activity loss is observed after storage at: Room temperature (RT) for 1 month in lyophilized state; -20℃ for 1 year in lyophilized state; -70℃ for 6 months under sterile conditions after reconstitution.

Assay Principles/原理

This assay kit employs a standard Indirect-ELISA format, which provides a rapid quantification of Anti-Her2 antibodies through antigen binding. The Kit includes of Streptavidin pre-coated plate, the Biotinylated Her2 protein, a human anti-Her2 antibody standard, a secondary HRP-anti-Human IgG antibody and an assay diluent.

Your experiment will only have 5 simple steps:

1. Coat the plate with Biotinylated Her2 protein. Block and wash the plate.

2. Add samples to the plate, and use human anti-Her2 antibody as control. Incubate and wash the plate.

3. Add the diluted secondary HRP-anti-human IgG, Fc antibody to the plate and incubate.

4.Wash the plate and then add TMB or other colorimetric HRP substrate.

5. Stop the substrate reaction by adding the diluted acid. Read OD at 450 nm, which reflects the amount of antibody bound.

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400-682-2521(全国)
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Her2靶点信息
英文全称:Receptor protein-tyrosine kinase erbB-2
中文全称:受体蛋白酪氨酸激酶 erbB-2
种类:Homo sapiens
上市药物数量:24详情
临床药物数量:126详情
最高研发阶段:批准上市
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