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 >  Protein>Streptavidin >STN-N5116

Recombinant Streptavidin Protein (MALS verified)

分子别名(Synonym)

Streptavidin,SA

表达区间及表达系统(Source)

Recombinant Streptavidin (STN-N5116) is expressed from E.coli cells.

蛋白结构(Molecular Characterization)

This protein carries no "tag". The protein has a calculated MW of 13.8 kDa.

应用说明(Application)

ELISA assay

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in NaCl, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 2 years in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Streptavidin SDS-PAGE

The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Streptavidin SEC-MALS

The purity of Recombinant Streptavidin (Cat. No. STN-N5116) is more than 95% and the molecular weight of this protein is around 54-60 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Streptavidin ELISA

Immobilized Biotinylated Human CD19 (20-291), His,Avitag (Cat. No. CD9-H82E9) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-Human CD19 Antibody, Mouse IgG2a with a linear range of 0.1-6 ng/mL (QC tested).

Protocol

Streptavidin ELISA

Immobilized Biotinylated SARS-CoV-2 S protein RBD, His,Avitag (Cat. No. SPD-C82E9) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 with a linear range of 0.1-4 ng/mL (Routinely tested).

Protocol

 

背景(Background)

Streptavidin is a tetrameric protein purified from the bacterium Streptomyces avidinii, and exhibits high binding affinity for biotin. Able to bind one molecule of biotin with each subunit. Streptavidin (PI=6.0-7.5) has lower level of non-specific binding to various biological components at physiological pH than avidin (PI=7.4), resulting from its isoelectric point (PI).Streptavidin is useful in affinity chromatography, ELISA, immunohistochemistry and Western Blotting.

文献引用(Citations)

 

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