膜杰作
Star Staining
分子别名（Synonym）
IL-31,IL31,Interleukin-31
表达区间及表达系统（Source）
Human IL-31, His Tag (IL1-H5247) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Thr 164 (Accession # Q6EBC2-1).
Predicted N-terminus: His
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 17.7 kDa. The protein migrates as 23-33 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human IL-31, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human IL-31, His Tag (Cat. No. IL1-H5247) is more than 90% and the molecular weight of this protein is around 15-25 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human IL-31, His Tag (Cat. No. IL1-H5247) at 1 μg/mL (100 μL/well) can bind Human IL-31 RA, Fc Tag (Cat. No. ILA-H5256) with a linear range of 0.02-0.625 μg/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Human IL-31, His Tag (Cat. No. IL1-H5247) immobilized on CM5 Chip can bind Human IL-31 RA, His Tag (Cat. No. ILA-H52H7) with an affinity constant of 52.1 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Human IL-31, His Tag (Cat. No. IL1-H5247) capture on NTA-Series S sensor chip can bind Human IL-31 RA, Fc Tag (Cat. No. ILA-H5256) with an affinity constant of 37.1 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
背景（Background）
IL-31 is an inflammatory cytokine that helps trigger cell-mediated immunity against pathogens. Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. It has also been identified as a major player in a number of chronic inflammatory diseases, including atopic dermatitis. May function in skin immunity. IL-31 is produced by a variety of cells, namely type 2 helper (TH2) T-cells. IL-31 sends signals through a receptor complex made of IL-31RA and oncostatin M receptor β (OSMRβ) expressed in immune and epithelial cells. These signals activate three pathways: ERK1/2 MAP kinase, PI3K/AKT, and JAK1/2 signaling pathways.
