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SARS-CoV-2 (COVID-19) Inhibitor screening Kit

For research use only.

IDComponentsSizeSize
A010-214SARS-CoV-2 Spike RBD10 μg60 μg
A011-214Biotinylated Human ACE210 μg10 μg
A003-214Streptavidin-HRP10 μg10 μg
AC2-NA005SARS-CoV-2 Inhibitor (Human ACE2)20 μg50 μg

Background/背景

The newly identified 2019 novel coronavirus (SARS-CoV-2) has posed a serious threat to human health. Currently, there is no specific treatment for COVID-19. It is urgent to develop the SARS-CoV-2 inhibitors, for example vaccines and therapeutic antibodies and small molecular compounds against COVID-19.

Application/应用

This kit is useful for screening inhibitors of SARS-CoV-2.

ELISA Buffer Set-96T (EBS-001) is sold separately and not included in kit, and please contact us if you need the buffer.

Reconstitution/重构方法

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage/存储

Upon receipt, please store all items at -20℃ to -70℃. After reconstitution, the stock solution should be kept at -70℃.

It is recommended not to freeze thaw more than 3 times.

This product is stable after storage at: Room temperature (RT) for 1 month in lyophilized state; ●-20℃ to -70℃ for 1 year in lyophilized state; ● -70℃ for 6 months under sterile conditions after reconstitution

Assay Principles/原理

This inhibitor screening ELISA pair is designed to facilitate the identification and characterization of SARS-CoV-2 inhibitors. This assay employs a simple colorimetric ELISA platform, which measures the binding between immobilized SARS-CoV-2 S protein RBD and in-house developed biotinylated Human ACE2 protein. This product is uniquely suitable for rapid high-throughput screening of SARS-CoV-2 inhibitors. Briefly, we provide you with a biotinylated Human ACE2 protein, a SARS-CoV-2 S protein RBD protein, an SARS-CoV-2 inhibitor (as method verified Reference), and Streptavidin-HRP reagent.

Your experiment will include 4 simple steps:

a) Coat the plate with SARS-CoV-2 S protein RBD.

b) Add 50ul biotinylated human ACE2 to coated plate.

c) Add the reference or your molecule of interest.

d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.

Finally, the ability of your compound to inhibit S protein RBD: ACE2 binding will be determined by comparing OD readings among different experimental groups.

 

Typical Data/典型数据

Biotinylated Human ELISA

Binding of Biotinylated Human ACE2 To Immobilized SARS-CoV-2 S protein RBD In A Functional ELISA Assay.
Immobilized 2019-CoV-2 S protein RBD at 0.5 μg/mL (100 μL/well) can bind human ACE2-Biotin with a linear range of 0.001- 0.06 μg/mL when detected by Streptavidin-HRP. Background was subtracted from data points before curve fitting.

Protocol

Biotinylated Human SPR

Inhibition of Human ACE2 [Biotinylated] :SARS-CoV-2 S protein RBD Binding by SARS-CoV-2 inhibitor.
Serial dilutions of SARS-CoV-2 inhibitor (Cat. No. AC2-NA005) (1:2 serial dilutions, from 20 μg/mL to 0.039 μg/mL (180 nM to 0.35 nM)) was added into Biotinylated ACE2: SARS-CoV-2 S protein RBD binding reactions. The assay was performed according to the above described protocol. Background was subtracted from data points prior to log transformation and curve fitting.

Protocol

文献引用(Citations)

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