膜杰作
Star Staining
分子别名（Synonym）
CCL19,MIP-3 beta,MIP-3-beta,ELC,MIP3B,SCYA19,EBI1 ligand chemokine
表达区间及表达系统（Source）
Human CCL19, Tag Free (CC9-H5215) is expressed from human 293 cells (HEK293). It contains AA Gly 22 - Ser 98 (Accession # Q99731-1).
Predicted N-terminus: Gly 22
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries no "tag"
The protein has a calculated MW of 8.8 kDa. The protein migrates as 12 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 0.1 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>95% as determined by SEC-HPLC.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human CCL19, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-HPLC
The purity of Human CCL19, Tag Free (Cat. No. CC9-H5215) was greater than 95% as determined by SEC-HPLC.
背景（Background）
C-C motif chemokine 19(CCL19) is as known as CK beta-11, ELC, MIP3B and SCYA19. May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
