膜杰作
Star Staining
分子别名（Synonym）
GUCY2C,GUC2C,STAR,STA receptor,hSTAR,GC-C
表达区间及表达系统（Source）
Human GUCY2C, His Tag (GUC-H52H5) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Gln 430 (Accession # P25092-1).
Predicted N-terminus: Ser 24
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 47.9 kDa. The protein migrates as 55-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human GUCY2C, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Human GUCY2C, His Tag (Cat. No. GUC-H52H5) is more than 90% and the molecular weight of this protein is around 58-78 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human GUCY2C, His Tag (Cat. No. GUC-H52H5) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human GUCY2C Antibody, Human IgG1 with a linear range of 0.3-5 ng/mL (QC tested).
Protocol
活性（Bioactivity）-FACS
2e5 of anti-GUCY2C CAR-293 cells were stained with 100 µL of 1 µg/mL Human GUCY2C, His Tag (Cat. No. GUC-H52H5) and negative control protein respectively, washed and then followed by PE anti-His antibody and analyzed with FACS (Routinely tested).
Protocol
背景（Background）
GUCY2C (Guanylyl Cyclase C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (gc) family that signal by producing cGMP. Guanylate cyclase C (GUCY2C) and its hormones guanylin and uroguanylin have recently emerged as one paracrine axis defending intestinal mucosal integrity against mutational, chemical, and inflammatory injury. GUCY2C murine CAR-T cells recognized and killed human colorectal cancer cells endogenously expressing GUCY2C. Thus, we have identified a human GUCY2C-specific CAR-T cell therapy approach that may be developed for the treatment of GUCY2C-expressing metastatic colorectal cancer.
