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 >  Protein>B7-H5 >B75-H5259

Human B7-H5 / Gi24 / VISTA Protein, Fc Tag

分子别名(Synonym)

B7-H5,SISP1,Gi24,VISTA

表达区间及表达系统(Source)

Human B7-H5, Fc Tag (B75-H5259) is expressed from human 293 cells (HEK293). It contains AA Phe 33 - Ala 194 (Accession # AAH20568 ).

Predicted N-terminus: Phe 33

Request for sequence

蛋白结构(Molecular Characterization)

B7-H5 Structure

This protein carries a human IgG1 Fc tag at the C-terminus

The protein has a calculated MW of 44.8 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

B7-H5 SDS-PAGE

Human B7-H5, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-SPR

B7-H5 SPR

Human PSGL-1, His Tag (Cat. No. PS1-H52H9) immobilized on CM5 Chip can bind Human B7-H5, Fc Tag (Cat. No. B75-H5259) with an affinity constant of 0.213 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

背景(Background)

Platelet receptor Gi24, also known as B7-H5 and stress-induced secreted protein-1 (Sisp-1), is a protein that in humans is encoded by the C10orf54 gene, which contains 1 Ig-like (immunoglobulin-like) domain. As for C10orf54 gene, C10orf54 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state on ESCs. Human C10orf54 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment.

 

前沿进展

 
 
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B7-H5靶点信息
英文全称:V-domain Ig suppressor of T-cell activation
中文全称:T细胞活化的免疫球蛋白抑制V型结构域
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:7详情
最高研发阶段:临床二期
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