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 >  Protein>PSGL-1 >PS1-H52H9

Human PSGL-1 / CD162 Protein, His Tag

分子别名(Synonym)

PSGL1,CD162,SELPLG,Selectin P ligand

表达区间及表达系统(Source)

Human PSGL-1, His Tag (PS1-H52H9) is expressed from human 293 cells (HEK293). It contains AA Gln 42 - Cys 320 (Accession # Q14242-1).

Predicted N-terminus: Gln 42

Request for sequence

蛋白结构(Molecular Characterization)

Online(Gln 42 - Cys 320) Q14242-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 30.7 kDa. The protein migrates as 55-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human PSGL-1, His Tag (Cat. No. PS1-H52H9) SDS-PAGE gel

Human PSGL-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

活性(Bioactivity)-SPR

Biotinylated Human SPR

Human PSGL-1, His Tag (Cat. No. PS1-H52H9) immobilized on CM5 Chip can bind Human B7-H5, Fc Tag (Cat. No. B75-H5259) with an affinity constant of 0.213 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

背景(Background)

P-selectin glycoprotein ligand 1 (PSGL-1) is also known as Selectin P ligand (SELPLG), CD antigen CD162. PSGL-1 is disulfide-linked homodimer which is the high affinitycounter-receptor for P-selectin on expressed on activated endothelial cells and platelets. PSGL-1 / SELPLG interacts with P-, E- and L-selectins, through their lectin/EGF domains, is required for promoting recruitment and rolling of leukocytes. These interactions require sialyl Lewis X glycan modification but there is a differing dependence for tyrosine sulfations. Dimerization appears not to be required for P-selectin/SELP binding. PSGL-1 can interact with SNX20, MSN and SYK. PSGL-1 / SELPLG mediate the activation of SYK by SELPLG.

 

 

前沿进展

 
 
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PSGL-1靶点信息
英文全称:P-selectin glycoprotein ligand-1
中文全称:P选择素糖蛋白配体1
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:4详情
最高研发阶段:临床二期
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