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 >  Protein>Serum Albumin >MSA-M52H8

Mouse Serum Albumin Protein, His Tag (MALS verified)

分子别名(Synonym)

Serum albumin,ALB,Alb

表达区间及表达系统(Source)

Mouse Serum Albumin, His Tag (MSA-M52H8) is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Ala 608 (Accession # P07724-1).

Predicted N-terminus: Glu 25

Request for sequence

蛋白结构(Molecular Characterization)

Online(Glu 25 - Ala 608) P07724-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 67.8 kDa. The protein migrates as 65-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Mouse Serum Albumin, His Tag (Cat. No. MSA-M52H8) SDS-PAGE gel

Mouse Serum Albumin, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Mouse Serum Albumin, His Tag (Cat. No. MSA-M52H8) MALS images

The purity of Mouse Serum Albumin, His Tag (Cat. No. MSA-M52H8) is more than 90% and the molecular weight of this protein is around 60-75 kDa verified by SEC-MALS.

Report

活性(Bioactivity)-SPR

Biotinylated Human SPR

Mouse Serum Albumin, His Tag (Cat. No. MSA-M52H8) immobilized on CM5 Chip can bind Mouse FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCM-M52W8) with an affinity constant of 0.165 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag (BLI verified)(Cat. No. FCM-M82W5) on SA Biosensor, can bind Mouse Serum Albumin, His Tag(Cat. No. MSA-M52H8) with an affinity constant of 0.495 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

serum albumin (SA) is also known as ALB, which is the main protein of plasma and has a good binding capacity for water,Ca2+,Na+,K+,fatty acids,hormones, bilirubin and drugs.The main function of SA is the regulation of the colloidal osmotic pressure of blood. As Major zinc transporter in plasma, SA typically binds about 80% of all plasma zinc. A variant structure of albumin could lead to increased binding of zinc resulting in an asymptomatic augmentation of zinc concentration in the blood. Defects in serum albumin can cause familial dysalbuminemic hyperthyroxinemia which is a form of euthyroid hyperthyroxinemia that is due to increased affinity of serum albumin for T4. It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.

 

 

前沿进展

 
 
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Serum Albumin靶点信息
英文全称:Serum albumin
中文全称:血清白蛋白
种类:Homo sapiens
上市药物数量:16详情
临床药物数量:14详情
最高研发阶段:批准上市
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