IL3R,IL3RA,IL-3Ra,IL-3R-alpha,IL3RAY,IL3RX,IL3RY,CD123 antigen,CD123,hIL3Ra,hIL-3Ra,MGC34174,IL-3 R alpha
Mouse IL-3 R alpha, His Tag (ILA-M52H4) is expressed from human 293 cells (HEK293). It contains AA Ser 17- Lys 331 (Accession # P26952-1).
Predicted N-terminus: Ser 17
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 36.4 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Mouse IL-3 R alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Mouse IL-3 R alpha, His Tag (Cat. No. ILA-M52H4) captured on CM5 Chip via anti-His antibody can bind Mouse IL-3, Tag Free with an affinity constant of 0.704 μM as determined in SPR assay (Biacore T200) (QC tested).
Loaded Mouse IL-3 R alpha, His Tag (Cat. No. ILA-M52H4) on NTA Biosensor, can bind Mouse IL-3, Tag Free with an affinity constant of 0.38 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Interleukin 3 receptor alpha (low affinity) (IL3RA), also known as CD123 (Cluster of Differentiation 123) is a 70-kD glycoprotein member of the hematopoietin receptor superfamily. This protein associates with a beta subunit common to the receptors for IL-5 and granulocyte-macrophage colony-stimulating factor (GM-CSF) to form a high-affinity receptor for IL-3. The interleukin-3 receptor α chain (CD123) has been identified as a potential immunotherapeutic target because it is overexpressed in AML compared with normal hematopoietic stem cells.