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 >  Protein>IL-3 R alpha >ILA-M52H4

Mouse IL-3 R alpha / CD123 Protein, His Tag (SPR verified)

分子别名(Synonym)

IL3R,IL3RA,IL-3Ra,IL-3R-alpha,IL3RAY,IL3RX,IL3RY,CD123 antigen,CD123,hIL3Ra,hIL-3Ra,MGC34174,IL-3 R alpha

表达区间及表达系统(Source)

Mouse IL-3 R alpha, His Tag (ILA-M52H4) is expressed from human 293 cells (HEK293). It contains AA Ser 17- Lys 331 (Accession # P26952-1).

Predicted N-terminus: Ser 17

Request for sequence

蛋白结构(Molecular Characterization)

Online(Ser 17- Lys 331) P26952-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 36.4 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Mouse IL-3 R alpha, His Tag (Cat. No. ILA-M52H4) SDS-PAGE gel

Mouse IL-3 R alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

活性(Bioactivity)-SPR

Biotinylated Human SPR

Immobilized Mouse IL-3 R alpha, His Tag (Cat. No. ILA-M52H4) on CM5 Chip can bind Mouse IL-3 Protein, His Tag (Cat. No. IL3-M52H8) with an affinity constant of 0.929 μM as determined in a SPR assay (Biacore T200) (QC tested).

Protocol

 

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Mouse IL-3 R alpha, His Tag (Cat. No. ILA-M52H4) on NTA Biosensor, can bind Mouse IL-3, Tag Free with an affinity constant of 0.38 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Interleukin 3 receptor alpha (low affinity) (IL3RA), also known as CD123 (Cluster of Differentiation 123) is a 70-kD glycoprotein member of the hematopoietin receptor superfamily. This protein associates with a beta subunit common to the receptors for IL-5 and granulocyte-macrophage colony-stimulating factor (GM-CSF) to form a high-affinity receptor for IL-3. The interleukin-3 receptor α chain (CD123) has been identified as a potential immunotherapeutic target because it is overexpressed in AML compared with normal hematopoietic stem cells.

 

 

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IL-3 R alpha靶点信息
英文全称:Interleukin-3 receptor subunit alpha
中文全称:白细胞介素-3受体α亚基
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:33详情
最高研发阶段:批准上市
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