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 >  Protein>Siglec-10 >SI0-H82F6

Biotinylated Human Siglec-10 Protein, Fc,Avitag™ (MALS verified)

分子别名(Synonym)

SIGLEC10,MGC126774,PRO940,Siglec10,SLG2

表达区间及表达系统(Source)

Biotinylated Human Siglec-10, Fc,Avitag (SI0-H82F6) is expressed from human 293 cells (HEK293). It contains AA Met 17 - Thr 546 (Accession # Q96LC7-1).

Predicted N-terminus: Met 17

Request for sequence

蛋白结构(Molecular Characterization)

Siglec-10 Structure

This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 86.4 kDa. The protein migrates as 100-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Siglec-10 SDS-PAGE

Biotinylated Human Siglec-10, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Siglec-10 SEC-MALS

The purity of Biotinylated Human Siglec-10, Fc,Avitag (Cat. No. SI0-H82F6) is more than 90% and the molecular weight of this protein is around 176-216 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Siglec-10 ELISA

Immobilized Biotinylated Human Siglec-10, Fc,Avitag (Cat. No. SI0-H82F6) at 5 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Anti-Human Siglec-10 MAb, human IgG with a linear range of 0.6-20 ng/mL (QC tested).

Protocol

 

背景(Background)

The siglecs (sialic acid-binding Ig-like lectins) are a distinct subset of the Ig superfamily with adhesion-molecule-like structure. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products.

 

前沿进展

 
 
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Siglec-10靶点信息
英文全称:Sialic acid-binding Ig-like lectin 10
中文全称:唾液酸结合免疫球蛋白样凝集素-10
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:4详情
最高研发阶段:临床三期
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