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 >  Protein>Nectin-4 >NE4-H82E7

Biotinylated Human Nectin-4 Protein, His,Avitag™ (MALS verified)

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分子别名(Synonym)

NECTIN4,LNIR, PRR4, PVRL4

表达区间及表达系统(Source)

Biotinylated Human Nectin-4, His,Avitag (NE4-H82E7) is expressed from human 293 cells (HEK293). It contains AA Gly 32 - Ser 349 (Accession # Q96NY8-1 ).

Predicted N-terminus: Gly 32

Request for sequence

蛋白结构(Molecular Characterization)

Nectin-4 Structure

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)

The protein has a calculated MW of 37.8 kDa. The protein migrates as 40-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Nectin-4 SDS-PAGE

Biotinylated Human Nectin-4, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Nectin-4 SEC-MALS

The purity of Biotinylated Human Nectin-4, His,Avitag (Cat. No. NE4-H82E7) is more than 90% and the molecular weight of this protein is around 38-56 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Nectin-4 ELISA

Immobilized Biotinylated Human Nectin-4, His,Avitag (Cat. No. NE4-H82E7) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Human Nectin-1, Fc Tag (Cat. No. PV1-H5253) with a linear range of 2-40 ng/mL (QC tested).

Protocol

Nectin-4 ELISA

Immobilized Biotinylated Human Nectin-4, His,Avitag (Cat. No. NE4-H82E7) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Mouse Anti-Nectin-4 Antibody with a linear range of 0.2-3 ng/mL (QC tested).

Protocol

 

背景(Background)

Nectin-4 (gene name PVRL4, poliovirus receptor-like 4) is a 66 kDa type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. Nectins are cell adhesion molecules that play a key role in various biological processes such as polarity, proliferation, differentiation and migration, for epithelial, endothelial, immune and neuronal cells, during development and adult life. Nectin-4 is a tumor-associated antigen in 50%, 49% and 86%o of breast, ovarian and lung carcinomas, respectively, mostly on tumors of bad prognosis. Its expression is not detected in the corresponding normal tissues.

 

前沿进展

 
 
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Nectin-4靶点信息
英文全称:Nectin-4
中文全称:细胞黏附分子nectin-4
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:8详情
最高研发阶段:批准上市
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