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 >  Protein>Complement C5a >C5A-H525a

Human Complement C5a Protein, Fc Tag (MALS verified)

分子别名(Synonym)

C5a,Complement Component 5a

表达区间及表达系统(Source)

Human Complement C5a, Fc Tag (C5A-H525a) is expressed from human 293 cells (HEK293). It contains AA Leu 679 - Arg 751 (Accession # P01031-1 ).

Predicted N-terminus: Leu 679

Request for sequence

蛋白结构(Molecular Characterization)

Complement C5a Structure

This protein carries a human IgG1 Fc tag at the C-terminus

The protein has a calculated MW of 34.6 kDa. The protein migrates as 38-40 kDa and 41-44 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Complement C5a SDS-PAGE

Human Complement C5a, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Complement C5a SEC-MALS

The purity of Human Complement C5a, Fc Tag (Cat. No. C5A-H525a) is more than 90% and the molecular weight of this protein is around 80-90 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Complement C5a ELISA

Immobilized Human Complement C5a, Fc Tag (Cat. No. C5A-H525a) at 2 μg/mL (100 μL/well) can bind Anti-C5a (Human IgG1) with a linear range of 2-16 ng/mL (QC tested).

Protocol

 

背景(Background)

Complement Component C5a (C5a) is also known as C5,and is a protein fragment released from complement component C5.,is a potent chemotactic factor for human peripheral blood neutrophils and monocytes, and is believed to play an important role in a number of inflammatory conditions. There are several functions in the below: C5a is an anaphylatoxin, causing the release of histamine from mast cells; C5a is effective leukocyte chemoattractants, causing the accumulation of white blood cells, especially neutrophil granulocytes, at sites of complement activation; C5a activates white blood cells by increasing avidity for white blood cell integrins and upregulating the Lipoxygenase pathway for arachidonic acid metabolism; C5a is a powerful inflammatory mediator, and seems to be a key factor in the development of pathology of many inflammatory diseases involving the complement system; C5a modulates balance between activating versus inhibitory IgG Fc receptors on leukocytes, thereby enhancing autoimmune response.

 

前沿进展

 
 
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Complement C5a靶点信息
英文全称:Complement C5a
中文全称:补体C5a
种类:
上市药物数量:0详情
临床药物数量:3详情
最高研发阶段:临床三期
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