登录 | 注册    关注公众号  
微信公众号
搜索
 >  Protein>Fc gamma RIV / CD16-2 >FC4-M82E8

Biotinylated Mouse Fc gamma RIV / CD16-2 Protein, His,Avitag™ (HPLC & BLI verified)

热销产品推荐:

利用生物素-链霉亲和素系统的高特异性和高灵敏度的结合特性,可将生物素化蛋白与链霉亲和素相关产品联合应用于各类实验,与非生物素体系相比,检测速度更快、结合能力更强、非特异背景更低。

分子别名(Synonym)

Fc gamma RIV,CD16-2,Fcgr4

表达区间及表达系统(Source)

Biotinylated Mouse Fc gamma RIV, His,Avitag (BLI verified) (FC4-M82E8) is expressed from human 293 cells (HEK293). It contains AA Gly 21 - Gln 203 (Accession # A0A0B4J1G0-1 ).

Predicted N-terminus: Gly 21

Request for sequence

蛋白结构(Molecular Characterization)

Fc gamma RIV / CD16-2 Structure

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)

The protein has a calculated MW of 24.6 kDa. The protein migrates as 30-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 12 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Fc gamma RIV / CD16-2 SDS-PAGE

Biotinylated Mouse Fc gamma RIV, His,Avitag (BLI verified) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-HPLC

Fc gamma RIV / CD16-2 SEC-HPLC

The purity of Biotinylated Mouse Fc gamma RIV, His,Avitag (BLI verified) (Cat. No. FC4-M82E8) was greater than 85% as determined by SEC-HPLC.

 

活性(Bioactivity)-ELISA

Fc gamma RIV / CD16-2 ELISA

Immobilized FMC63 at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse Fc gamma RIV, His,Avitag (BLI verified) (Cat. No. FC4-M82E8) with a linear range of 0.3-20 ng/mL (Routinely tested).

Protocol

 

活性(Bioactivity)-SPR

Fc gamma RIV / CD16-2 SPR

Biotinylated Mouse Fc gamma RIV, His,Avitag (BLI verified) (Cat. No. FC4-M82E8) captured on Biotin CAP - Series S sensor Chip can bind Herceptin with an affinity constant of 0.186 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Protocol

Fc gamma RIV / CD16-2 SPR

Biotinylated Mouse Fc gamma RIV, His,Avitag (BLI verified) (Cat. No. FC4-M82E8) captured on Biotin CAP - Series S sensor Chip can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), Ultra-low endotoxin (Cat. No. CDE-M120a) with an affinity constant of 31.7 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

Fc gamma RIV / CD16-2 BLI

Biotinylated Mouse Fc gamma RIV, His,Avitag (BLI verified) (Cat. No. FC4-M82E8) on SA Biosensor, can bind Rituximab with an affinity constant of 0.394 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

Protocol

背景(Background)

FcgR4(Low affinity immunoglobulin gamma Fc region receptor IV) is also known as CD16-2, FcgammaRIV, receptor for the Fc region of immunoglobulin gamma . Also acts as a receptor for the Fc region of immunoglobulin epsilon . Binds with intermediate affinity to both IgG2a and IgG2b . Does not display binding to IgG1 or IgG3. Plays a role in promoting bone resorption by enhancing osteoclast differentiation following binding to IgG2a. Binds with low affinity to both the a and b allotypes of IgE. Has also been shown to bind to IgE allotype a only but not to allotype b. Binding to IgE promotes macrophage-mediated phagocytosis, antigen presentation to T cells, production of proinflammatory cytokines and the late phase of cutaneous allergic reactions

 

文献引用(Citations)

 

前沿进展

 
 
货号/价格
产品推荐
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
Fc gamma RIV / CD16-2靶点信息
英文全称:P-selectin glycoprotein ligand-1
中文全称:P选择素糖蛋白配体1
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:4详情
最高研发阶段:临床二期
查看更多信息
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定