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 >  Protein>IL-33 >IL3-H52H7

Human IL-33 Protein, His Tag, premium grade

分子别名(Synonym)

IL33,DV27,C9ORF26,IL1F11,NFHEV,DKFZp586H0523,DVS27,NFEHEV,RP11-575C20.2

表达区间及表达系统(Source)

Human IL-33 Protein, His Tag, premium grade (IL3-H52H7) is expressed from human 293 cells (HEK293). It contains AA His 109 - Thr 270 (Accession # O95760-1).

Predicted N-terminus: His

Human IL-33 Protein, His Tag, premium grade (IL3-H52H7), designed for preclinical stage, has the same activity and performance with GMP Human IL-33 Protein, which enables a seamless transition from preclinical development to clinical phases. Premium Grade product offer a cost efficient alternative of GMP Grade products for the early development phase when safety of raw materials is not top priority. By using Premium Grade products in early development phase, you can transition easily into clinical and commercial phase without need to revalidate the raw materials and modify manufacturing process.

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蛋白结构(Molecular Characterization)

IL-33 Structure

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 20.2 kDa. The protein migrates as 25-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

无菌(Sterility)

The sterility testing was performed by membrane filtration method.

支原体(Mycoplasma)

Negative.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

IL-33 SDS-PAGE

Human IL-33 Protein, His Tag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS

IL-33 SEC-MALS

The purity of Human IL-33 Protein, His Tag, premium grade (Cat. No. IL3-H52H7) is more than 85% and the molecular weight of this protein is around 18-26 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-Bioactivity CELL BASE

IL-33 CELL

Human IL-33 Protein, His Tag, premium grade (Cat. No. IL3-H52H7) induces IL-8 secretion in HUVECs. The EC50 for this effect is 193.0-311.8 ng/mL (Routinely tested).

Protocol

 

活性(Bioactivity)-ELISA

IL-33 ELISA

Immobilized Human IL-1RL1, Fc Tag (Cat. No. IL1-H5250) at 5 μg/mL (100 μL/well) can bind Human IL-33 Protein, His Tag, premium grade (Cat. No. IL3-H52H7) with a linear range of 5-40 ng/mL (QC tested).

Protocol

 

活性(Bioactivity)-BLI

IL-33 BLI

Loaded Human IL-1RL1, Fc Tag (Cat. No. IL1-H5250) on Protein A Biosensor, can bind with Human IL-33 Protein, His Tag, premium grade (Cat. No. IL3-H52H7) an affinity constant of 1.31 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Interleukin 33 (IL33) is known as C9orf26, DKFZp586H0523, DVS27, NF-HEV, NFEHEV, RP11-575C20.2,and is a cytokine belonging to the IL-1 superfamily. IL-33 induces helper T cells, mast cells, eosinophils and basophils to produce type 2 cytokines. IL-33 mediates its biological effects by interacting with the receptors ST2 (aka IL1RL1) and IL-1 Receptor Accessory Protein (IL1RAP), activating intracellular molecules in the NF-κB and MAP kinase signaling pathways that drive production of type 2 cytokines (e.g. IL-5 and IL-13) from polarized Th2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs.

 

前沿进展

 
 
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IL-33靶点信息
英文全称:Interleukin-33
中文全称:白细胞介素-33
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:10详情
最高研发阶段:临床三期
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