FITC-Labeled Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-HF25x) is expressed from human HEK293 cells. It contains AA Glu 296 - Gly 580 (Accession # AAH09272.1 ). It is the FITC labeled form of Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-H526x).
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This protein carries a human IgG1 Fc tag at the N-terminus
The protein has a calculated MW of 59.1 kDa. The protein migrates as 60-67 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.
The FITC to protein molar ratio is 1-2.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
FITC-Labeled Human Mesothelin (296-580), Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Anti-Human MSLN antibody at 10 μg/mL (100 μL/well) can bind FITC-Labeled Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-HF25x) with a linear range of 0.039-0.625 μg/mL (QC tested).
2e5 of anti MSLN-CAR-293 cells transfected with Anti-MSLN scfv were stained with 100 μL of 3 μg/mL of FITC-Labeled Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-HF25x) and negative control protein respectively, FITC signal was used to evaluate the binding activity (QC tested).
Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.