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 >  Protein>IL-12 R beta 1 >ILB-H5255

Human IL-12 R beta 1 / CD212 Protein, Fc Tag

分子别名(Synonym)

IL-12 R beta 1,IL12RB1,CD212,IL12R,IL12RB,IL-12RB1

表达区间及表达系统(Source)

Human IL-12 R beta 1, Fc Tag (ILB-H5255) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Glu 540 (Accession # P42701-1 ).

Predicted N-terminus: Cys 24

Request for sequence

蛋白结构(Molecular Characterization)

IL-12 R beta 1 Structure

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 83.5 kDa. The protein migrates as 95-120 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

IL-12 R beta 1 SDS-PAGE

Human IL-12 R beta 1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

 

活性(Bioactivity)-ELISA

IL-12 R beta 1 ELISA

Immobilized Human IL-12B, His Tag (Cat. No. NK2-H52H7) at 5 μg/mL (100 μL/well) can bind Human IL-12 R beta 1, Fc Tag (Cat. No. ILB-H5255) with a linear range of 5-78 ng/mL (QC tested).

Protocol

 

活性(Bioactivity)-SPR

IL-12 R beta 1 SPR

Human IL-12 R beta 1, Fc Tag (Cat. No. ILB-H5255) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tag (Cat. No. IL2-H4210) with an affinity constant of 2.06 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

IL-12 R beta 1 BLI

Loaded Human IL-12 R beta 1, Fc Tag (Cat. No. ILB-H5255) on Protein A Biosensor, can bind Human IL-12B, His Tag (Cat. No. NK2-H52H7) with an affinity constant of 7.14 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

IL-12 R beta 1 BLI

Loaded Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tag (Cat. No. IL2-H4210) on HIS1K Biosensor, can bind Human IL-12 R beta 1, Fc Tag (Cat. No. ILB-H5255) with an affinity constant of 32.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

The human IL-12 R subunit is a member of the cytokine receptor superfamily and the functional high-affinity IL-12R is composed of at least two beta-type cytokine receptor subunits, each independently exhibiting a low affinity for IL-12. IL-12 R beta 1 (Interleukin-12 receptor subunit beta-1) is also known as IL-12RB1, CD212. Functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.

 

前沿进展

 
 
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IL-12 R beta 1靶点信息
英文全称:Interleukin-12 receptor
中文全称:白细胞介素-12受体
种类:
上市药物数量:0详情
临床药物数量:10详情
最高研发阶段:临床三期
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