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 >  Protein>TRAIL R2 >TR2-M52H5

Mouse TRAIL R2 / DR5 / TNFRSF10B Protein, His Tag

分子别名(Synonym)

TNFRSF10B,TRAILR2,TRAIL-R2,CD262,DR5,KILLER,TRICK2,ZTNFR9,TRICKB

表达区间及表达系统(Source)

Mouse TRAIL R2, His Tag (TR2-M52H5) is expressed from human 293 cells (HEK293). It contains AA Asn 53 - Lys 180 (Accession # Q9QZM4-1).

Predicted N-terminus: Asn 53

Request for sequence

蛋白结构(Molecular Characterization)

TRAIL R2 Structure

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 16.1 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

TRAIL R2 SDS-PAGE

Mouse TRAIL R2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

TRAIL R2 ELISA

Immobilized Human TNFSF10 at 2 μg/mL (100 μL/well) can bind Mouse TRAIL R2, His Tag (Cat. No. TR2-M52H5) with a linear range of 0.039-0.625 μg/mL (QC tested).

Protocol

 

背景(Background)

Tumor necrosis factor receptor superfamily member 10B (TNFRSF10B) is also known as TNF-related apoptosis-inducing ligand receptor 2 (TRAILR2), Death receptor 5 (DR5), CD262, KILLER, is a member of the TNF-receptor superfamily, and contains an intracellular death domain. TNFRSF10B / DR-5 is widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines. TRAILR2 / CD262 / DR5 is the receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD (a death domain containing adaptor protein) of TRAIL-R2 / TNFRSF10B recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. CD262 / DR5 Promotes the activation of NF-kappa-B. DR5 is essential for ER stress-induced apoptosis and is regulated by p53/TP53.

 

前沿进展

 
 
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TRAIL R2靶点信息
英文全称:Tumor necrosis factor receptor superfamily member 10B
中文全称:肿瘤坏死因子受体超家族成员10B
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:11详情
最高研发阶段:批准上市
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