Human ADAM8, His Tag (AD8-H5223) is expressed from human 293 cells (HEK293). It contains AA Ile 17 - Pro 497 (Accession # AAI15405.1).
Predicted N-terminus: Ile 17
Human ADAM8, His Tag fused with a polyhistidine tag at the C-terminus, has a calculated MW of 53.8 kDa, which migrates as a 130-150 kDa band in DTT-reduced SDS-PAGE due to glycosylation. Two main fragments bands are visible with apparent MW of 58-68 kDa and 35-40 kDa respectively in reduced SDS-PAGE as a result of its being prone to proteolytic cleavage.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human ADAM8, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Disintegrin and metalloproteinase domain-containing protein 8 is also known as ADAM8, Cell surface antigen MS2 and CD antigen CD156a, which belongs to the ADAM (a disintegrin and metalloprotease domain) family. ADAM family play a fundamental role in diverse processes such as asthma, development, angiogenesis and cancer through their activities in cell adhesion/fusion, membrane protein shedding, and signal transduction. ADAM8 / CD156a contains 1 disintegrin domain, 1 EGF-like domain and 1 peptidase M12B domain. ADAM8 / MS2 / CD156a is expressed on neutrophils and monocytes. ADAM8 / MS2 possible involvement in extravasation of leukocytes.