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 >  Protein>Fc gamma RIV / CD16-2 >FC4-M52H3

Mouse Fc gamma RIV / CD16-2 Protein, His Tag (MALS verified)

分子别名(Synonym)

Fc gamma RIV,CD16-2,Fcgr4,FCGR4,mouse igg,Fc receptor

表达区间及表达系统(Source)

Mouse CD16-2, His Tag (FC4-M52H3) is expressed from human 293 cells (HEK293). It contains AA Gly 21 - Gln 203 (Accession # A0A0B4J1G0-1).

Predicted N-terminus: Gly 21

Request for sequence

蛋白结构(Molecular Characterization)

Online(Gly 21 - Gln 203) A0A0B4J1G0-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 22.8 kDa. The protein migrates as 28-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 12 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Mouse CD16-2, His Tag (Cat. No. FC4-M52H3) SDS-PAGE gel

Mouse CD16-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Mouse CD16-2, His Tag (Cat. No. FC4-M52H3) MALS images

The purity of Mouse CD16-2, His Tag (Cat. No. FC4-M52H3) is more than 90% and the molecular weight of this protein is around 25-35 kDa verified by SEC-MALS.

Report

活性(Bioactivity)-SPR

Biotinylated Human SPR

Captured Mouse CD16-2, His Tag (Cat. No. FC4-M52H3) on NTA-Series S sensor chip can bind OKT3 with an affinity constant of 154 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Mouse CD16-2, His Tag (Cat. No. FC4-M52H3) on NTA Biosensor, can bind OKT3 with an affinity constant of 140 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Loaded Mouse CD16-2, His Tag (Cat. No. FC4-M52H3) on NTA Biosensor, can bind Herceptin with an affinity constant of 122 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

文献引用(Citations)

背景(Background)

FcgR4(Low affinity immunoglobulin gamma Fc region receptor IV) is also known as CD16-2, FcgammaRIV, receptor for the Fc region of immunoglobulin gamma . Also acts as a receptor for the Fc region of immunoglobulin epsilon . Binds with intermediate affinity to both IgG2a and IgG2b . Does not display binding to IgG1 or IgG3. Plays a role in promoting bone resorption by enhancing osteoclast differentiation following binding to IgG2a. Binds with low affinity to both the a and b allotypes of IgE. Has also been shown to bind to IgE allotype a only but not to allotype b. Binding to IgE promotes macrophage-mediated phagocytosis, antigen presentation to T cells, production of proinflammatory cytokines and the late phase of cutaneous allergic reactions

 

文献引用(Citations)

 

前沿进展

 
 
Fc gamma RIV / CD16-2靶点信息
英文全称:P-selectin glycoprotein ligand-1
中文全称:P选择素糖蛋白配体1
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:4详情
最高研发阶段:临床二期
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