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FITC-Labeled Human SLAMF7 / CRACC / CD319 Protein, His Tag

分子别名(Synonym)

SLAMF7,CD319,CS1,CRACC,19A,FOAP-12

表达区间及表达系统(Source)

FITC-Labeled Human SLAMF7, His Tag (Cat. No. SL7-HF2H7) is expressed from human HEK293 cells. It contains AA Ser 23 - Met 226 (Accession # AAH27867). It is the FITC labeled form of Human SLAMF7, His Tag (Cat. No. SL7-H5225).

Predicted N-terminus: Ser 23

蛋白结构(Molecular Characterization)

Online(Ser 23 - Met 226) AAH27867

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 23.2 kDa. The protein migrates as 33-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Conjugate

FITC

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 535 nm

标记(Labeling)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular seive treatment during purification process.

蛋白标记度(FITC:Protein Ratio)

The FITC to protein molar ratio is 3-4.5.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

FITC-Labeled Human SLAMF7, His Tag (Cat. No. SL7-HF2H7) SDS-PAGE gel

FITC-Labeled Human SLAMF7, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

活性(Bioactivity)-FACS

Biotinylated Human FACS

2e5 of Anti-SLAMF7 CAR-293 cells were stained with 100 μL of 10 μg/mL of FITC-Labeled Human SLAMF7, His Tag (Cat. No. SL7-HF2H7) and negative control protein respectively, FITC signal was used to evaluate the binding activity (QC tested)

Protocol

 

背景(Background)

SLAM family member 7 (SLAMF7) is also known as CD2-like receptor-activating cytotoxic cells (CRACC), Membrane protein FOAP-12, CD antigen CD319, Novel Ly9, Protein 19A, which is a single-pass type I membrane protein and a member of the CD2 family of cell surface receptors. SLAMF7 is expressed in spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung and trachea. Isoform 1 of SLAMF7 mediates NK cell activation through a SH2D1A-independent extracellular signal-regulated ERK-mediated pathway. May play a role in lymphocyte adhesion. Isoform 3 of SLAMF7 does not mediate any NK cell activation.

 

前沿进展

 
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SLAMF7靶点信息
英文全称:SLAM family member 7
中文全称:SLAM家族成员-7
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:4详情
最高研发阶段:批准上市
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