登录 | 注册    关注公众号  
微信公众号
搜索
 >  Protein>IL-2 R alpha >ILA-H82E6

Biotinylated Human IL-2 R alpha / CD25 Protein, His,Avitag™, premium grade

热销产品推荐:

利用生物素-链霉亲和素系统的高特异性和高灵敏度的结合特性,可将生物素化蛋白与链霉亲和素相关产品联合应用于各类实验,与非生物素体系相比,检测速度更快、结合能力更强、非特异背景更低。

分子别名(Synonym)

IL2RA,CD25,p55,IL2-RA,IL-2-RA

表达区间及表达系统(Source)

Biotinylated Human IL-2 R alpha, His,Avitag, premium grade (ILA-H82E6) is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Cys 213 (Accession # P01589-1).

Predicted N-terminus: Glu 22

Biotinylated Human IL-2 R alpha, His,Avitag, premium grade (ILA-H82E6), designed for preclinical stage, has the same activity and performance with GMP Biotinylated Human IL-2 R alpha, which enables a seamless transition from preclinical development to clinical phases. Premium Grade product offer a cost efficient alternative of GMP Grade products for the early development phase when safety of raw materials is not top priority. By using Premium Grade products in early development phase, you can transition easily into clinical and commercial phase without need to revalidate the raw materials and modify manufacturing process.

Request for sequence

蛋白结构(Molecular Characterization)

IL-2 R alpha Structure

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)

The protein has a calculated MW of 25.4 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

无菌(Sterility)

The sterility testing was performed by membrane filtration method.

支原体(Mycoplasma)

Negative.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

IL-2 R alpha SDS-PAGE

Biotinylated Human IL-2 R alpha, His,Avitag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

IL-2 R alpha SEC-MALS

The purity of Biotinylated Human IL-2 R alpha, His,Avitag, premium grade (Cat. No. ILA-H82E6) is more than 90% and the molecular weight of this protein is around 25-40 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-Bioactivity CELL BASE

IL-2 R alpha CELL

Biotinylated Human IL-2 R alpha, His,Avitag, premium grade (Cat. No. ILA-H82E6) inhibits the IL-2-dependent proliferation of Mo7e cells. The EC50 for this effect is 0.57-0.81 µg/mL (Routinely tested).

Protocol

 

活性(Bioactivity)-ELISA

IL-2 R alpha ELISA

Immobilized Human IL-2, Tag Free at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-2 R alpha, His,Avitag, premium grade (Cat. No. ILA-H82E6) with a linear range of 0.02-1 μg/mL (QC tested).

Protocol

 

活性(Bioactivity)-BLI

IL-2 R alpha BLI

Loaded Biotinylated Human IL-2 R alpha, His,Avitag, premium grade (Cat. No. ILA-H82E6) on SA Biosensor, can bind Human IL-2, Tag Free with an affinity constant of 70.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Interleukin-2 receptor subunit alpha (IL2RA) is also known as IL-2R subunit alpha, IL-2-RA, IL2-RA, TAC antigen, p55, CD antigen CD25, is a type I transmembrane glycoprotein. IL2RA is expressed on activated T cells and regulatory T cells, and is capable of binding IL2 with low affinity by itself. However, a ligand-induced high affinity heterotrimeric receptor complex is produced when IL2RA is associated non-covelently with the IL2 receptor beta and gamma chain, and subsequently initiates the intacellular signal pathways such as MAPK or JAK/STAT. On dendritic cells (DC), CD25 has been previously regarded as an activation marker, while both murine and human DC can express CD25, they do not express the beta-chain of the IL-2 receptor, which is indispensable for the execution of IL-2 signaling.

 

前沿进展

 
 
货号/价格
产品推荐
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
IL-2 R alpha靶点信息
英文全称:Interleukin-2 receptor alpha chain
中文全称:白细胞介素-2受体α链
种类:Homo sapiens
上市药物数量:3详情
临床药物数量:14详情
最高研发阶段:批准上市
查看更多信息
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定