膜杰作
Star Staining
ViruStop
Aneuro
ComboX
ActiveMax
GENPower
ClinMax
分子别名（Synonym）
PDGF-BB,PDGF-B,FLJ12858,PDGF2,SIS,SSV,c-sis
表达区间及表达系统（Source）
Human PDGF-BB, Tag Free (PDB-H4112) is expressed from E.coli cells. It contains AA Ser 82 - Thr 190 (Accession # P01127-1).
Predicted N-terminus: Met
Request for sequence
蛋白结构（Molecular Characterization）
Human PDGF-BB, Tag Free is a disulfide-linked homodimeric polypeptide chain containing 2×109 amino acids, and has a calculated MW of 12.4 kDa. The reducing (R) protein migrates as a 14 kDa polypeptide and the non-reducing (NR) disulfide-linked homodimer migrates as a 27-32 kDa protein.
内毒素（Endotoxin）
Less than 0.1 EU per μg by the LAL method.
无菌（Sterility）
The sterility testing was performed by membrane filtration method described in USP <71> and Eur. Ph. 2.6.1.
纯度（Purity）
>90% as determined by reducing (R) and non-reducing (NR) SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 0.085% TFA in 30% ACN. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human PDGF-BB, Tag Free on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
活性（Bioactivity）-ELISA
Immobilized Human PDGF-BB, Tag Free (Cat. No. PDB-H4112) at 1 μg/mL (100 μL/well) can bind Human PDGF R beta, Fc Tag (Cat. No. PDB-H5259) with a linear range of 0.6-10 ng/mL (QC tested).
Protocol
活性（Bioactivity）-Cell based assay
The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse 3T3 cells. The ED50 was 0.5-20 ng/mL (Routinely tested).
Protocol
文献引用（Citations）
Authors: Hao Han, et al.
Journal: Biochem Bioph Res Co 2018
Application: Cell Culture
背景（Background）
PDGFs are mitogenic during early developmental stages, driving the proliferation of undifferentiated mesenchyme and some progenitor populations. During later maturation stages, PDGF signalling has been implicated in tissue remodelling and cellular differentiation, and in inductive events involved in patterning and morphogenesis. In addition to driving mesenchymal proliferation, PDGFs have been shown to direct the migration, differentiation and function of a variety of specialised mesenchymal and migratory cell types, both during development and in the adult animal. Other growth factors in this family include vascular endothelial growth factors B and C (VEGF-B, VEGF-C)which are active in angiogenesis and endothelial cell growth, and placenta growth factor (PlGF) which is also active in angiogenesis. PDGF plays a role in embryonic development, cell proliferation, cell migration, and angiogenesis. PDGF is a required element in cellular division for fibroblast, a type of connective tissue cell. PDGF is also known to maintain proliferation of oligodendrocyte progenitor cells. Platelet-derived growth factor subunit B is also known as PDGFB, FLJ12858, PDGF2, SIS, SSV, c-sis, is a member of the platelet-derived growth factor family. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma.
