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 >  Antibody>Adalimumab >ADB-Y19

Anti-Adalimumab Antibody (AY19)

抗体来源(Source)

Mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Adalimumab F(ab')2.

亚型(Isotype)

Mouse IgG1/kappa

特异性(Specificity)

Recognizes Adalimumab specifically, no cross reactivity with other humanized antibodies.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 12 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Adalimumab SDS-PAGE

Anti-Adalimumab Antibody (AY19) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Adalimumab ELISA

Anti-Adalimumab Antibodies bridging ELISA for Anti-Drug Antibody (ADA) assay development.Immobilized adalimumab at 1 µg/mL, add increasing concentrations of Anti-Adalimumab Antibody (AY19) (Cat. No. ADB-Y19, 10% human serum) and then add biotinylated adalimumab at 5 µg/mL. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 0.6 ng/mL.

Protocol

Adalimumab ELISA

Demonstration of the specificity of Anti-Adalimumab Antibody (AY19) (Cat. No. ADB-Y19) to the adalimumab.

Protocol

Adalimumab ELISA

Measured by its neutralizing ability in a functional ELISA. Immobilized adalimumab at 0.5 μg/mL (100 μL/well) can bind pre-mixed Anti-Adalimumab Antibody (AY19) (Cat. No. ADB-Y19) and Biotinylated Human TNF-alpha, His,Avitag (Cat. No. TNA-H82E3) with an inhibition rate of 100%.

Protocol

 

活性(Bioactivity)-SPR

Adalimumab SPR

Anti-Adalimumab Antibody (AY19) (mouse IgG1, Cat. No. ADB-Y19) captured on CM5 chip via anti-mouse antibodies surface, can bind human adalimumab with an affinity constant of 1.36 pM.

Protocol

 

背景(Background)

Adalimumab is a human monoclonal antibody against TNF-alpha. It is produced by recombinant DNA technology using a mammalian cell expression system. It consists of 1330 amino acids and has a molecular weight of approximately 148 kilodaltons.

 

前沿进展

 
 
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