B7-H7,HHLA2,B7 Homolog 7
Human B7-H7, His Tag (B77-H52H5) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Asn 344 (Accession # Q9UM44-1).
Predicted N-terminus: Ile 23
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 38.9 kDa. The protein migrates as 48-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human B7-H7, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human CD28H, Fc Tag (Cat. No. CDH-H5251) at 5 μg/mL (100 μL/well) can bind Human B7-H7, His Tag (Cat. No. B77-H52H5) with a linear range of 0.02-0.313 μg/mL (QC tested).
Immobilized Human B7-H7, His Tag (Cat. No. B77-H52H5) at 5 μg/mL (100 μL/well) can bind Human CD28H, Fc Tag (Cat. No. CDH-H5251) with a linear range of 1-20 ng/mL (Routinely tested).
B7-H7 (HHLA2) is a newly identified B7 family member that regulates human T-cell functions. B7-H7 was previously known as human endogenous retrovirus-H long terminal repeat associating 2 (HHLA2) with unidentified function. Recently, B7-H7 has been identified as a specific ligand for human CD28H. The B7-H7-CD28H pathway strongly promoted CD4+ T-cell proliferation and cytokine production via an AKT-dependent signaling cascade in the presence of TCR signaling, suggesting B7-H7 comprises a new co-stimulatory pathway. The first IgV domain of B7-H7, which presumably binds to a putative receptor, shows the highest homology to other B7 family members.